- Phosphopeptide-dependent labeling of 14-3-3 ζ proteins by fusicoccin-based fluorescent probes.
Phosphopeptide-dependent labeling of 14-3-3 ζ proteins by fusicoccin-based fluorescent probes.
Angewandte Chemie (International ed. in English) (2011-11-23)
Michiko Takahashi, Akie Kawamura, Nobuo Kato, Tsuyoshi Nishi, Itaru Hamachi, Junko Ohkanda
PMID22105970
RÉSUMÉ
Fluorescent combination: Cell-penetrating probes derived from the diterpene fusicoccin can form ternary complexes with 14-3-3 proteins and phosphopeptide ligands, whereupon the probes site-specifically attach a fluorescent tag onto the surface of the 14-3-3 proteins.
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