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Recombinant protein production by in vivo polymer inclusion display.

Applied and environmental microbiology (2011-08-02)
Katrin Grage, Verena Peters, Bernd H A Rehm
RÉSUMÉ

A novel approach to produce purified recombinant proteins was established. The target protein is produced as polyhydroxyalkanoate (PHA) synthase fusion protein, which mediates intracellular formation of PHA inclusions displaying the target protein. After isolation of the PHA inclusions, the pure target protein was released by simple enterokinase digestion.

MATÉRIAUX
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Description du produit

Sigma-Aldrich
Enterokinase from bovine intestine, powder
Sigma-Aldrich
Enterokinase from porcine intestine, ≥0.5 units/mg solid
Sigma-Aldrich
Enterokinase from porcine intestine, lyophilized powder, ≥100 units/mg protein