Accéder au contenu
MilliporeSigma
  • Isolation and molecular characterization of multiresistant Staphylococcus sciuri and Staphylococcus haemolyticus associated with skin and soft-tissue infections.

Isolation and molecular characterization of multiresistant Staphylococcus sciuri and Staphylococcus haemolyticus associated with skin and soft-tissue infections.

Journal of medical microbiology (2003-12-10)
Adebayo Shittu, Johnson Lin, Donald Morrison, Deboye Kolawole
RÉSUMÉ

The isolation, molecular identification and genotyping of multiresistant Staphylococcus sciuri and Staphylococcus haemolyticus from skin and soft-tissue infections are reported. Accurate and full identification of three coagulase-negative staphylococcal isolates was achieved using PCR, while the API STAPH method failed to identify an isolate of S. haemolyticus fully. The PCR assay, which detects polymorphism in the 16S-23S rRNA spacer region, is shown to be potentially useful for rapid and accurate identification of coagulase-negative staphylococci. Identical PFGE type and antibiotic-resistance profiles of two methicillin-resistant S. haemolyticus isolates in this study suggest the existence of a multiresistant community clone.

MATÉRIAUX
Référence du produit
Marque
Description du produit

Sigma-Aldrich
Achromopeptidase from bacteria, lyophilized powder, ≥1,000 units/mg solid
Sigma-Aldrich
Achromopeptidase from Lysobacter enzymogenes, partially purified powder, ≥20,000 units/mg solid