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Synthesis of bioinspired collagen/alginate/fibrin based hydrogels for soft tissue engineering.

Materials science & engineering. C, Materials for biological applications (2018-07-24)
G Montalbano, S Toumpaniari, A Popov, P Duan, J Chen, K Dalgarno, W E Scott, A M Ferreira
RÉSUMÉ

Hydrogels based on natural polymers offer a range of properties to mimic the native extracellular matrix, and provide microenvironments to preserve cellular function and encourage tissue formation. A tri-component hydrogel using collagen, alginate and fibrin (CAF) was developed and investigated at three collagen concentrations for application as a functional extracellular matrix analogue. Physical-chemical characterization of CAF hydrogels demonstrated a thermo-responsive crosslinking capacity at physiological conditions with stiffness similar to native soft tissues. CAF hydrogels were also assessed for cytocompatibility using L929 murine fibroblasts, pancreatic MIN6 β-cells and human mesenchymal stem cells (hMSCs); and demonstrated good cell viability, proliferation and metabolic activity after 7 days of in vitro culture. CAF hydrogels, especially with 2.5% w/v collagen, increased alkaline phosphatase production in hMSCs indicating potential for the promotion of osteogenic activity. Moreover, CAF hydrogels also increased metabolic activity of MIN6 β-cells and promoted the reconstitution of spherical pseudoislets with sizes ranging between 50 and 150 μm at day 7, demonstrating potential in diabetic therapeutic applications.

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Description du produit

Sigma-Aldrich
Acide acétique, glacial, ReagentPlus®, ≥99%
Sigma-Aldrich
Fibrinogène from bovine plasma, Type I-S, 65-85% protein (≥75% of protein is clottable)
Sigma-Aldrich
Thrombine from bovine plasma, lyophilized powder, 40-300 NIH units/mg protein (biuret)
Sigma-Aldrich
Alginic acid sodium salt from brown algae, low viscosity