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Determination of ticlopidine in human plasma by high-performance liquid chromatography and ultraviolet absorbance detection.

Journal of chromatography. B, Biomedical applications (1995-03-24)
L Dal Bo, F Verga, A Marzo, L Ambrosoli, A Poli
RÉSUMÉ

A simple HPLC method has been developed for the determination of ticlopidine in human plasma. Plasma samples were buffered at pH 9 and extracted with n-heptane-isoamyl alcohol (98.5:1.5, v/v). Imipramine was used as internal standard. Chromatography was performed isocratically with acetonitrile-methanol-0.05 M KH2PO4 (20:25:55, v/v) at pH 3.0 containing 3% triethylamine at a flow-rate of 1 ml/min. A reversed-phase column, Supelcosil LC-8-DB, 15 cm x 4.6 mm I.D., 5 microns particle size, was used. The effluent was monitored by UV absorbance detection at 235 nm. The method showed good accuracy, precision and linearity in the concentration range 5-1200 ng/ml. The limit of quantitation was 5 ng/ml, with a precision (C.V.) of 8.91%, which is the same as that achieved by other authors with a previously published GC-MS method. The procedure described in this paper is simple and allows the routine assessment of ticlopidine plasma concentration in pharmacokinetic studies following therapeutic doses in human subjects.

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Supelco
Colonne HPLC SUPELCOSIL LC-8-DB, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
Supelco
Colonne HPLC SUPELCOSIL LC-8-DB, 5 μm particle size, L × I.D. 15 cm × 4.6 mm
Supelco
Colonne HPLC SUPELCOSILLC-8, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
Supelco
Colonne HPLC SUPELCOSIL LC-NH2-NP, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
Supelco
Colonne HPLC SUPELCOSILLC-8, 3 μm particle size, L × I.D. 7.5 cm × 4.6 mm
Supelco
Colonne HPLC SUPELCOSIL LC-8-DB, 3 μm particle size, L × I.D. 15 cm × 4.6 mm
Supelco
Colonne HPLC SUPELCOSILLC-8, 5 μm particle size, L × I.D. 15 cm × 4.6 mm
Supelco
Colonne HPLC SUPELCOSILLC-8, 3 μm particle size, L × I.D. 15 cm × 4.6 mm
Supelco
Colonne HPLC SUPELCOSILLC-8, 5 μm particle size, L × I.D. 5 cm × 4.6 mm
Supelco
Colonne HPLC SUPELCOSILLC-8, 5 μm particle size, L × I.D. 25 cm × 10 mm
Supelco
Cartouches SUPELCOSIL LC-8-DB Supelguard, 5 μm particle size, L × I.D. 2 cm × 3 mm
Supelco
Colonne HPLC SUPELCOSILLC-8, 3 μm particle size, L × I.D. 3.3 cm × 4.6 mm
Supelco
Colonne HPLC SUPELCOSILLC-8, 5 μm particle size, L × I.D. 15 cm × 4 mm
Supelco
Colonne HPLC SUPELCOSIL LC-8-DB, 5 μm particle size, L × I.D. 5 cm × 4.6 mm
Supelco
Cartouche SUPELCOSIL LC-8 Supelguard, 5 μm particle size, L × I.D. 2 cm × 4 mm
Supelco
Colonne HPLC SUPELCOSIL LC-8-DB, 5 μm particle size, L × I.D. 25 cm × 4 mm
Supelco
Cartouche SUPELCOSIL LC-8 Supelguard, 5 μm particle size, L × I.D. 2 cm × 2.1 mm
Supelco
Colonne HPLC SUPELCOSIL LC-8-DB, 3 μm particle size, L × I.D. 3.3 cm × 4.6 mm
Supelco
Cartouches SUPELCOSIL LC-8-DB Supelguard, 5 μm particle size, L × I.D. 2 cm × 4 mm
Supelco
Cartouche Supelguard SUPELCOSIL LC-MH2-NP, 5 μm particle size, L × I.D. 2 cm × 4 mm
Supelco
Cartouche SUPELCOSIL LC-8 Supelguard, 5 μm particle size, L × I.D. 2 cm × 4 mm
Supelco
Colonne HPLC SUPELCOSIL LC-8-DB, 3 μm particle size, L × I.D. 3.3 cm × 2.1 mm
Supelco
Cartouches SUPELCOSIL LC-8-DB Supelguard, 5 μm particle size, L × I.D. 2 cm × 4 mm