Accéder au contenu
MilliporeSigma
HomeProductsProtein BiologyProteins, Enzymes & Small MoleculesDetection Substrates & Enzymes

Detection Substrates & Enzymes

Enzyme-based protein detection systems for ELISA, immunohistochemistry, western blotting, and many more with our diverse portfolio of detection substrates and enzymes

Detection substrates and enzymes are crucial in many areas of life science, both as tools and targets in detection systems. Our enzyme substrates and substrate detection systems offer convenience, stability, and consistency. They are routinely analyzed in our laboratories for identity, assay, and homogeneity, as well as for functional performance in the corresponding enzyme system. We offer substrates, buffers, and reagents in powder, liquid, tablet, and capsule forms for use in enzyme assays, ELISA, blotting, imaging, and many other applications. Our high purity enzymes have undergone extensive characterization and offer lot-to-lot consistency. Explore our enzyme products and discover ways to utilize our detection substrates and enzymes in different detection methods.

Related Product Literature

Substrate Systems for Enzyme Detection

Peroxidase Enzymes

Our peroxidase enzymes are recognized around the world as the industry standard for diagnostic manufacturing and laboratory-scale research applications. Horseradish peroxidase (HRP) enzymes, peroxidase enzyme products, horseradish peroxidase substrates, and inhibitors are widely used as secondary detection reagents in ELISAs, immuno-histochemical techniques, and blotting analyses. In addition, you can conjugate our HRP to your own molecule including antibodies and avidins. Our peroxidase products include variations of HRP enzyme and chromogenic substrates of HRP such as 3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA and SIGMAFAST™ OPD (o-Phenylenediamine dihydrochloride) tablet. Other products include chemiluminescence substrates of HRP for ultra-sensitive detection such as Lumi-LightPLUS Western Blotting Substrate and BM Chemiluminescence ELISA Substrate (POD).

Alkaline Phosphatase (ALP) Enzymes

Our reliable ALPs are widely used in immunohistochemical assays, northern, Southern, and western blot applications, as well as ELISA-based diagnostics. Explore our ALP resource to find the right protocol for your assay needs. We offer a variety of ALP products and alkaline phosphatase substrates. Options include colorimetric substrates and chromogenic substrates such as phosphatase substrate tablets, SIGMAFAST™ BCIP®/NBT tablet, and Alkaline Phosphatase Yellow (pNPP) Liquid Substrate System for ELISA ready-to-use solution. Histochemical substrates include naphthol AS-TR phosphate disodium salt and 5-bromo-4-chloro-3-indolyl phosphate disodium salt. Additionally, we provide chemiluminescence substrates for ultra-sensitive detection such as CDP-Star®, ready-to-use disodium 2-chloro-5-(4-methoxyspiro {1,2-dioxetane-3,2′-(5′chloro)tricyclo [3.3.1.13,7 ] decan}-4-yl)-1-phenyl phosphate from Roche.

Luciferase Reagents

Luciferase is the original source for bioluminescent technology and firefly luciferase is a widely used bioluminescent reporter. These highly sensitive luciferase enzyme products are used extensively in molecular and cell biology, and for the efficient detection and quantitation of ATP as a reporter for genetic function. The light-producing reaction is initiated by D-luciferin activation. ATP is hydrolyzed and light is emitted when firefly luciferase catalyzes the oxidation of D-luciferin. We provide multiple formats of luciferase such as solutions and powders to fit your bioluminescent experiment needs. To protect the firefly population, we offer highly active green recombinant firefly luciferase. We also offer variations of the luciferase substrate, D-luciferin for luciferase assays.

Additional Detection Substrates

Our broad collection of additional detection substrates has numerous applications to suit your needs. This includes substrates for staining and labeling of actin microfilaments and the detection of cholesterol and lipoproteins in cells. Explore enzyme-based detection systems for ELISA, immunohistochemistry, and Western blotting including OPD, TMB, BCIP, pNPP, fast red, ABTS, DAB, chemiluminescence, and many more. We manufacture basic reagent powders as well as convenient solutions and tablets. We also offer enhancers and stop solutions specifically formulated for optimal performance with our detection substrates.

Related Product Categories
Molecular Cloning & Protein Expression
Molecular cloning and protein expression methods and protocols for reliable cloning and robust protein expression from insect, bacteria, and mammalian protein expression vectors.
ZooMAb® Antibodies
Experience ZooMAb® recombinant antibodies, engineered for exceptional consistency and performance in various applications.
Prestige Antibodies® Powered by Atlas Antibodies
Explore our Prestige Antibodies®, which are developed using open-access data from the Human Protein Atlas and validated using IHC, WB, ICC-IF, or RNA sequencing and compared to bioinformatic information and literature.
Small Pack Size Antibodies
Antibodies in 25 μg or 25 μL small pack sizes for your immunocytochemistry (ICC), immunohistochemistry (IHC), immunofluorescence (IF), immunoprecipitation (IP), flow cytometry, ELISA and western blot applications.
Enhanced Validation Antibodies
Our enhanced validation antibodies are characterized using numerous molecular and cell-based techniques and assays to provided superior assurance with target specificity for your required application needs.
Related Applications
Nucleic Acid Labeling & Detection
DNA and RNA nucleic acid labeling and detection methods and applications for northern blot, Southern blot, and additional nucleic acid detection assays.
Cloning & Expression
Cloning, protein expression vectors and techniques for optimal gene cloning and protein expression systems.
Gel Electrophoresis
Protein gel electrophoresis is used to separate proteins for purification, characterization, and detection. Methods such as native PAGE, SDS-PAGE, 2D PAGE, and isoelectric focusing (IEF) are used in preparation for downstream applications including Western blotting, mass spectrometry, and proteomic analysis.
Western Blotting
An overview of the principles and applications of Western blotting for protein detection. Includes links to detailed protocols, videos, and other resources for protein extraction, gel electrophoresis, transfer to PVDF or nitrocellulose membranes, and chemiluminescent, colorimetric, and fluorescent detection methods.
(ELISA) Enzyme-Linked Immunosorbent Assay
Enzyme-linked immunosorbent assay (ELISA) include direct, indirect, and capture (sandwich) detection formats for identifying and quantifying a specific antigen.
Immunohistochemistry
Immunohistochemistry (IHC) is a common immunostaining technique that is used by researchers to identify specific antigens of interest in healthy and diseased tissues.
Enzyme Activity Assays
Enzyme activity assay reagents and protocols for investigating the specific activity of an enzyme in an organism, tissue, or sample.
Protein Labeling & Modification
Protein labeling applications using enzyme-protein labeling, fluorescent protein labeling, and targeted protein degradation technologies.
Connectez-vous pour continuer

Pour continuer à lire, veuillez vous connecter à votre compte ou en créer un.

Vous n'avez pas de compte ?