Accéder au contenu
MilliporeSigma
HomeProductschemistry-and-biochemistryBiochemistryAntibiotics

Antibiotics

Illustration of molecules

The use of antibiotics in cell cultures prevents contamination from pathogens that induce morphological or physiological changes in cell cultivation as well as contamination of cell lines. Antibiotics, such as penicillin, puromycin, and ampicillin, are molecules that specifically target and kill cells by inhibiting or regulating cell-wall synthesis, nucleic acid metabolism and protein synthesis, or disrupting membrane structure.

We offer a wide variety of effective antibiotics, antibiotic extraction kits, and antibiotic ready-made solutions in varying concentrations for reliable results in your lab. Our antibacterial, antiviral, antifungal, and antineoplastic agents control and eradicate cell culture contamination to maintain aseptic conditions in your lab. Moreover, certain antibiotics also function as selection agents to establish transfected/genetically modified cells depending on your research needs.

In order to assist you in selecting the right antibiotics for your cell contamination prevention, genetic marker selection and cell biology studies, the Antibiotic Selection Guide provides application information, activity spectrum, and usage data. Derived from our expertise, peer-reviewed literature, and quality control analyses, we support you in determining suitable antibiotic concentrations to avoid toxicity and preserve your stock of irreplaceable cell cultures.

Explore our extensive portfolio of reliable antibiotics to keep your focus on creating breakthroughs.

Related Articles & Protocols
Antibiotics in Plant Tissue Culture Protocol
Reference guide and preparation guide for antibiotic and antimycotic use in plant tissue culture.
Antibiotic Optimization for Cell Transduction Using a Cytotoxicity Profile
Experimentally determine the appropriate antibiotic concentration for selecting stable cell lines. This titration protocol can determine the lowest concentration of puromycin needed to efficiently select transduced cells.
Why Use Antibiotics in Cell Culture?
Know when to use antibiotics to prevent bacterial or fungal, mycoplasma, or viral contamination in cell culture and find suitable antibiotics or other biological agents.
Inhibition of Cell Wall Biosynthesis by Antibiotics
Inhibition of Cell Wall Biosynthesis by Antibiotics
Inhibition of Protein Synthesis by Antibiotics
Protein synthesis is a complex, multi-step process involving many enzymes as well as conformational alignment. However, the majority of antibiotics that block bacterial protein synthesis interfere with the processes at the 30S subunit or 50S subunit of the 70S bacterial ribosome.
Related Product Categories
Classical Media & Buffers
Choose from a range of optimized cell culture media formulations, including DMEM, RPMI-1640, and serum-free options for diverse cellular needs.
Mammalian Cell Lines
Cell lines from human and other mammalian species are essential for modeling disease and biological systems, and are critical tools for protein, antibody, virus, and vaccine production. We offer authenticated, contaminant-free cell lines, many in partnership with ECACC.
Plant Culture Reagents
Plant tissue culture reagents, including gelling agents, plant growth regulators, auxins, cytokinins, and other supplements for supporting the growth and development of plant cells and tissues in culture.
Related Applications
Cell Culture Media Preparation
Culture media preparation for in vitro cell cultivation including preparation of different media formats, sterilization methods, cell growth and regulation supplements, media storage and use.
Cell & Developmental Biology Research
Rooted in physiology and biochemistry, cell and development biology features an interdisciplinary approach to understand intracellular and extracellular dynamics.
Cancer Research
Cancer research strives to not only identify the cause, but also methods for detection, treatment, and prevention. Learn more about our contributions to cancer research in academic and industrial settings.
Microbial Cell Culture
An overview of microbiological cell culture and its applications in molecular biology, cloning, and recombinant protein expression as well as cultivation for the isolation, differentiation, and identification of disease-causing microbes in clinical researc
Mammalian Cell Culture
Applications, principles, and methods for in vitro mammalian cell culture including links to articles, protocols, and videos for proper aseptic technique, passaging and subculturing, and culture media considerations.
Cell Growth & Maintenance
A synopsis of the conditions, media, nutrients, cultureware, and environments required for successful propagation of mammalian and yeast cells. Nutrient reagents, sterile technique and filtration, and requirements for 2D, 3D, and suspension cultures are described.
Cell Culture Troubleshooting
Expert tips for correcting common problems with cell culture including bacterial and fungal contamination, debris in culture media, cell detachment or clumping, slow growth, and low viability.
Related Services
Critical Raw Materials
Our customized portfolio of high-quality critical raw materials for API synthesis supplies the solvents, reagents, documentation, and expertise you need to meet stringent regulatory requirements and mitigate risk in all aspects of small molecule drug development and manufacturing.
Custom Cell Culture Media for Commercial Manufacturing
Connectez-vous pour continuer

Pour continuer à lire, veuillez vous connecter à votre compte ou en créer un.

Vous n'avez pas de compte ?