T8453

Anti-β-Tubulin II antibody, Mouse monoclonal

clone 7B9, purified from hybridoma cell culture

• Synonyme(s) : Anti-TUBB2, Anti-Tubulin, β2
• Numéro MDL: MFCD00145892
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Conjugué: unconjugated
• Forme d'anticorps: purified from hybridoma cell culture
• Type de produit anticorps: primary antibodies
• Clone: 7B9, monoclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen ~50 kDa
• Espèces réactives: human, mouse, rat
• Concentration: ~1.0 mg/mL
• Technique(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: 1-2 μg/mL using Neuro-2a cell extract
• Isotype: IgG1
• Numéro d'accès UniProt: Q9BVA1
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... TUBB2B(347733) ; mouse ... Tubb2b(73710) ; rat ... Tubb2b(291081)

Description

Description générale

Monoclonal Anti- β-Tubulin II (mouse IgG1 isotype) is derived from the hybridoma 7B9 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment at the C-terminus of human β?tubulin II. Tubulin is the major building block of microtubules. This intracellular, cylindrical, filamentous structure is present in almost all eukaryotic cells. Tubulin is a heterodimer that consists of α-tubulin and β ?tubulin. Both subunits have a molecular mass of ~50 kDa and share considerable homology.

Application

Anti-β-Tubulin II antibody has been used in

• immunoblotting immunoprecipitation
• immunocytochemistry
• western blotting
• immunolabelling

Actions biochimiques/physiologiques

The αβ tubulins are involved in the formation of microtubules during cell division by the formation of protofilaments.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)