SAB4200777
Anti-LbCas12a (Cpf1) antibody, Mouse monoclonal
clone LbCpf1, purified from hybridoma cell culture
Synonyme(s) :
Anti-CRISPR-associated endonuclease AsCas12a from Lachnospiraceae bacterium ND2006, Anti-LbCpf1 antibody, Mouse monoclonal
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About This Item
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified from hybridoma cell culture
Type de produit anticorps
primary antibodies
Clone
LbCpf1, monoclonal
Forme
buffered aqueous solution
Poids mol.
~135 kDa
Concentration
~1.0 mg/mL
Technique(s)
immunoblotting: 0.6-1.2 μg/mL using human HEK-293T cells over-expressing LbCpf1 protein
immunofluorescence: 0.25-0.5 μg/mL using human HEK-293T cells over-expressing LbCpf1 protein
immunoprecipitation (IP): 1-2.5 μg/test using lysate of human HEK-293T cells over-expressing LbCpf1 protein
Isotype
IgG1
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
Description générale
Clustered, regularly interspaced, short palindromic repeat (CRISPR) systems encode RNA-guided endonucleases that are essential for bacterial adaptive immunity. Depending on the architecture of the effector-CRISPR RNA (crRNA) interference module, different CRISPR-Cas systems could be assigned into two classes1: class 1 systems of multi-subunit complex, such as Cascade and class 2 systems of single enzyme, such as Cas9.
Cpf1 (CRISPR from Prevotella and Francisella 1) belongs to class 2 type V CRISPR-Cas endonuclease system. Cpf1 comprise several differences from Cas9 protein including cleavage with 5′ overhangs, a shorter guide RNA and a longer distance between the seed sequence and cleavage site.
LbCpf1, Cpf1 from Lachnospiraceae bacterium ND2006, was examined together with 15 members of Cpf1 nuclease family and proved to mediate efficient genome editing in HEK293FT cells with improved results compared to SpCas9.5 According to thr crystal structure, LbCpf1 has a triangle-shaped architecture with a large positively charged channel at the centre.7 The crRNA binding was shown to induce the pronounced structural rearrangements of LbCpf1, leading to formation of a substrate-binding conformation of LbCpf1. Over-expressed in plant cells, LbCpf1 demonstrated more than 10-fold transcriptional repression of the target gene.
Monoclonal anti-LbCpf1 antibody can provide a useful tool for genome editing research, such as detecting and monitoring LbCpf1 positively transfected cells.
Cpf1 (CRISPR from Prevotella and Francisella 1) belongs to class 2 type V CRISPR-Cas endonuclease system. Cpf1 comprise several differences from Cas9 protein including cleavage with 5′ overhangs, a shorter guide RNA and a longer distance between the seed sequence and cleavage site.
LbCpf1, Cpf1 from Lachnospiraceae bacterium ND2006, was examined together with 15 members of Cpf1 nuclease family and proved to mediate efficient genome editing in HEK293FT cells with improved results compared to SpCas9.5 According to thr crystal structure, LbCpf1 has a triangle-shaped architecture with a large positively charged channel at the centre.7 The crRNA binding was shown to induce the pronounced structural rearrangements of LbCpf1, leading to formation of a substrate-binding conformation of LbCpf1. Over-expressed in plant cells, LbCpf1 demonstrated more than 10-fold transcriptional repression of the target gene.
Monoclonal anti-LbCpf1 antibody can provide a useful tool for genome editing research, such as detecting and monitoring LbCpf1 positively transfected cells.
Immunogène
Recombinant LbCpf1
Application
Monoclonal Anti-LbCpf1 specifically recognizes Cpf1 from Lachnospiraceae bacterium ND2006. The product may be used in several immunochemical techniques including Immunoblotting (∼135 kDa), Immunofluorescence and Immunoprecipitation. Monoclonal Anti-LbCpf1 does not cross react with Cpf1 from Acidaminococcus sp. (strain BV3L6) ), SpCas9 from Streptococcus pyogenes bacteria and FnCas9 from Francisella novicida bacteria.
Forme physique
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Stockage et stabilité
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Autres remarques
This product is for R&D use only, not for drug, household, or other uses.
In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.
In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
nwg
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Xu Tang et al.
Nature plants, 3, 17018-17018 (2017-02-18)
Clustered regularly interspaced short palindromic repeats (CRISPR)-Cpf1 has emerged as an effective genome editing tool in animals. Here we compare the activity of Cpf1 from Acidaminococcus sp. BV3L6 (As) and Lachnospiraceae bacterium ND2006 (Lb) in plants, using a dual RNA
De Dong et al.
Nature, 532(7600), 522-526 (2016-04-21)
The CRISPR-Cas systems, as exemplified by CRISPR-Cas9, are RNA-guided adaptive immune systems used by bacteria and archaea to defend against viral infection. The CRISPR-Cpf1 system, a new class 2 CRISPR-Cas system, mediates robust DNA interference in human cells. Although functionally
Giedrius Gasiunas et al.
Proceedings of the National Academy of Sciences of the United States of America, 109(39), E2579-E2586 (2012-09-06)
Clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems provide adaptive immunity against viruses and plasmids in bacteria and archaea. The silencing of invading nucleic acids is executed by ribonucleoprotein complexes preloaded with small, interfering CRISPR RNAs (crRNAs) that act
Hui K Kim et al.
Nature methods, 14(2), 153-159 (2016-12-20)
CRISPR from Prevotella and Francisella 1 (Cpf1) is an effector endonuclease of the class 2 CRISPR-Cas (clustered regularly interspaced short palindromic repeats-CRISPR-associated proteins) gene editing system. We developed a method for evaluating Cpf1 activity, based on target sequence composition in
Eva Schunder et al.
International journal of medical microbiology : IJMM, 303(2), 51-60 (2013-01-22)
Francisella tularensis is a zoonotic agent and the subspecies novicida is proposed to be a water-associated bacterium. The intracellular pathogen F. tularensis causes tularemia in humans and is known for its potential to be used as a biological threat. We
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