R506-05N

Rat Lung Fibroblasts: RLF, neonatal

• Code UNSPSC : 41106514
• Nomenclature NACRES : NA.81

Propriétés

• Source biologique: rat lung
• Niveau de qualité: 100
• Conditionnement: pkg of 500,000 cells
• Fabricant/nom de marque: Cell Applications, Inc
• Mode de croissance: Adherent
• Morphologie: fibroblast
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): asthma; tuberculosis; cancer; cardiovascular diseases
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Description générale

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RLF from Cell Applications, Inc. have been used to demonstrate that exaggerated (compared to adults) NF-κB activation in neonates in response to hyperoxia is mediated by phosphorylation of IκBα on Tyr42 (Wright, 2009). They were also used to elucidate melatonin effects and signaling pathway (Ben Soussia, 2012) and served as a control in studies investigating epithelial-mesenchimal transition as the source of myofibroblasts responsible for idiopathic pulmonary fibrosis (Willis, 2005; Jain, 2007).

Origine de la lignée cellulaire

Lungs

Application

Pulmonary function, gene expression, healing & repair process, differentiation, extracellular matrix production, growth factor and cytokine activation, actin reorganization, synthesis of type II collagen, elastin, and proteoglycans, airway remodeling, pharmaceutical development.

Composants

Rat Fibroblast Basal Medium that contains 10% FBS and 10% DMSO

Qualité

Each lot was tested for proper morphology, Population Doublings, Negative for HIV, Hepatitis B, Hepatitis C, mycoplasma, bacteria, and fungi.

Notes préparatoires

• 2nd passage, >500,000 cells in Rat Fibroblast Basal Medium that contains 10% FBS and 10% DMSO
• Can be cultured at least 10 population doublings

Procédure de repiquage

Please refer to the RLF Culture Protocol.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable