P2165
Protein A–Biotin from Staphylococcus aureus
lyophilized powder
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About This Item
Produits recommandés
Conjugué
biotin conjugate
Niveau de qualité
Forme
lyophilized powder
Composition
Protein, ≥70% biuret
Ampleur du marquage
3.0-8.0 mol biotin per mol Protein A
Température de stockage
2-8°C
Description générale
Protein A (SpA) is a 42kDa virulence factor produced by Staphylococcus aureus, and is composed of five highly homologous consecutive extracellular Ig-binding domains, namely domains E, D, A, B, and C. This protein is present in both secreted and membrane-associated forms.
Spécificité
Binds IgG only from most mammals, except rat, goat, and sheep
Actions biochimiques/physiologiques
Protein A (SpA) causes B cells death in vitro, by binding to the Fc region of antibody and the Fab regions of the B-cell receptor, thus inhibiting its opsonophagocytosis. This protein is thought to be implicated in the evasion of host immune responses by Staphylococcus aureus, and thus, might be a candidate for vaccine development approaches to prevent severe S. aureus infections. S. aureus is responsible for nosocomial infections as well as a variety of human infections, such as food poisoning, toxic shock syndrome, endocarditis, septicemia, skin infections, soft tissue infections, and bone infections. It is also responsible for bovine and ovine mastitis. SpA typing or DNA sequence analysis of the variable repeat region of protein A gene is useful in determining S. aureus outbreak strains from those strains that are epidemiologically insignificant.
Forme physique
Lyophilized powder containing sodium citrate
Notes préparatoires
Prepared from P 6031, coupled to biotin by an amide bond through an aminocaproyl spacer.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Staphylococcus aureus produces a virulence factor, protein A (SpA), that contains five homologous Ig-binding domains. The interactions of SpA with the Fab region of membrane-anchored Igs can stimulate a large fraction of B cells, contributing to lymphocyte clonal selection. To
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