M2520

Medium 199

HEPES Modification, with Earle′s salts, L-glutamine and 25 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture

• Synonyme(s) : M199 Medium, TCM 199
• Code UNSPSC : 12352207
• Nomenclature NACRES : NA.75

Propriétés

• Niveau de qualité: 500
• Forme: powder
• Technique(s): cell culture | mammalian: suitable
• Composants: L-glutamine: yes; phenol red: yes; Earle’s salts (5% CO₂): yes; NaHCO₃: no; sodium pyruvate: no; HEPES: 25 mM
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C

Description

Description générale

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Application

Medium 199 has been used to:

• maintain the cumulus-oocyte complex (COC) before maturation and for culturing of these oocytes for in vitro maturation
• to rinse the aspiration line during the collection of the ovum from German Holstein cows
• to culture cardiac endothelial cells from rats

Quantité

Formulated to contain 14.7 grams of powder per liter of medium.

Reconstitution

Supplement with 2.2 g/L sodium bicarbonate.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable