GERPN1051-2ML
Streptavidin-Biotinylated HRP Complex
Cytiva RPN1051-2ML
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About This Item
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46
Produits recommandés
Conditionnement
pkg of 2 mL
Fabricant/nom de marque
Cytiva RPN1051-2ML
Couleur
Colorless
Conditions d'expédition
wet ice
Température de stockage
2-8°C
Catégories apparentées
Description générale
Streptavidin-Biotinylated Horseradish Peroxidase Complex.
Application
The conjugation of horseradish peroxidase and streptavidin is achieved using the periodate coupling method described by Nakane and coworkers. This product is sold on the basis of titer in an ELISA and performance in a Western blot. As a result the final Protein concentration of the conjugate (RPN 1231) will vary only minimally between batches.
Biotin is linked via a spacer arm to horseradish peroxidase under conditions that preserve high enzyme activity and give a high degree of biotinylation as judged by its ability to bind to streptavidin. Excess labeling reagent is removed by gel filtration.
Biotinylated enzyme is added to streptavidin to form complexes. The complex is optimized to produce an excellent combination of titer and sensitivity for binding to biotinylated Protein. This product is sold on the basis of titer determined in a standard assay. As a result the final Protein concentration of the complex may vary minimally between batches.
Biotin is linked via a spacer arm to horseradish peroxidase under conditions that preserve high enzyme activity and give a high degree of biotinylation as judged by its ability to bind to streptavidin. Excess labeling reagent is removed by gel filtration.
Biotinylated enzyme is added to streptavidin to form complexes. The complex is optimized to produce an excellent combination of titer and sensitivity for binding to biotinylated Protein. This product is sold on the basis of titer determined in a standard assay. As a result the final Protein concentration of the complex may vary minimally between batches.
Caractéristiques et avantages
- Streptavidin conjugated to reporter enzyme molecules for use in blotting and in situ hybridization applications.
- High specificity reagents.
Stockage et stabilité
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Remarque sur l'analyse
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Code de la classe de stockage
12 - Non Combustible Liquids
Certificats d'analyse (COA)
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