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Key Documents

GE28-9909-46

Sigma-Aldrich

Superdex® 200 Increase 3.2/300

column L × I.D. 30 cm × 3.2 mm, 8.6 μm particle size

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About This Item

Code UNSPSC :
41115700
Nomenclature NACRES :
SB.52

Matériaux

glass column
titanium frit

Gamme de produits

Superdex®

Durée de conservation

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Poids mol.

10000-600000 Da (globular proteins)

Conditionnement

1 ea of

Paramètres

<0.15 ml/min flow rate
3.0 Mpa (652 psi)
4-50 mL sample volume

Volume du lit

~2.4 mL

Colonne, L × D.I.

30 cm × 3.2 mm

Matrice

agarose-dextran composite
polymeric

Taille des particules

8.6 μm

cleaning

1-14

working range

3-12

Technique de séparation

size exclusion (SEC)

Catégories apparentées

Description générale

Superdex 200 is a column for high resolution gel filtration in micro scale (μg) preparative purification as well as characterization and analysis of small sample volumes (4-50 μL) of proteins with molecular weights between 10 000 and 600 000, such as antibodies. Pre-packed with small rigid agarose beads for high flow rates and pH stability.

Publication

Superdex Increase 200 is a column for high resolution gel filtration in small scale (mg) preparative purification as well as characterization and analysis of proteins with molecular weights between 10,000 and 600,000, such as antibodies. Pre-packed with small rigid agarose beads for high flow rates and pH stability.

Adéquation

Long term use: all commonly used aqueous buffers, up to 8 M urea, ionic and non-ionic detergents, up to 6M guanidine hydrochloride
for both preparative and analytical purposes

Stockage et stabilité

Store at 4 to 30 °C (20% Ethanol)

Produits recommandés

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Informations légales

Superdex is a registered trademark of Cytiva

Pictogrammes

Flame
GHS02

Mention d'avertissement

Warning

Mentions de danger

H226

Code de la classe de stockage

3 - Flammable liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Sigma-Aldrich

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Articles

Setting Column Pressure Limits for Size Exclusion Chromatography

Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system.

Protocoles

Performing a Separation with Superdex

Superdex from GE Healthcare are SEC media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superdex column.

Sample Preparation for Size Exclusion Chromatography

Samples for chromatographic purification should be clear and free from particulate matter. Simple steps to clarify a sample before beginning purification will avoid clogging the column, can reduce the need for stringent washing procedures, and can extend the life of the chromatographic medium.

Sample Preparation for Size Exclusion Chromatography

Samples for chromatographic purification should be clear and free from particulate matter. Simple steps to clarify a sample before beginning purification will avoid clogging the column, can reduce the need for stringent washing procedures, and can extend the life of the chromatographic medium.

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