GE17-5163-01

HiTrap^® Anx Fast Flow (high sub)

Cytiva 17-5163-01, pack of 5 × 5 mL

• Code UNSPSC : 23151816
• Nomenclature NACRES : NA.56

Propriétés

• ligand: diethylaminopropyl
• Description: Ion Exchanger Type (value)
• Conditionnement: pack of 5 × 5 mL
• Fabricant/nom de marque: Cytiva 17-5163-01
• Paramètres: 42 psi
• Taille du lit: 16 mm × 25 mm
• Volume du lit: 5 mL
• D.I. de la colonne: 16 mm
• Matrice: 4% cross-linked agarose
• Taille des particules: 45-165 μm
• Diamètre moyen: 90 μm
• cleaning: 2-14
• working range: 3-13
• Adéquation: suitable for bioprocess medium

Description

Description générale

HiTrap^® ANX (high sub) FF is prepacked with ANX (high sub) Sepharose^™ Fast Flow, and is a weak anion exchanger for small scale purification of higher molecular weight protein as well as screening of binding and elutions conditions.

Application

Q, SP, DEAE, and CM Sepharose^™ Fast FLow are based on a robust, 6% highly cross-linked beaded Agarose matrix with good flow properties and high loading capacities. ANX Sepharose^™ 4 Fast FLow (high sub) is based on 4% highly cross-linked beaded Agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass Proteins.

The active end of the charged group is the same for DEAE Sepharose Fast FLow and ANX Sepharose^™ Fast FLow (high sub), the difference is the length of the carbon chain of the charged group. DEAE Sepharose Fast FLow has a diethylaminoethyl-group bound to the Agarose whilst ANX Sepharose^™ 4 Fast FLow (high sub) has a diethylaminopropyl group attached.

Caractéristiques et avantages

• Convenient and affordable for fast, easy ion exchange separations either alone or connected in series.
• The industry standard for ion exchange chromatography.
• High fLow rates and good scale-up potential.
• Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient.
• Predictable scale-up

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Autres remarques

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Informations légales

HiTrap is a registered trademark of Cytiva

Sepharose is a trademark of Cytiva

Informations sur la sécurité

• Pictogrammes: GHS02
• Mention d'avertissement: Warning
• Mentions de danger: H226
• Conseils de prudence: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Code de la classe de stockage: 3 - Flammable liquids