GE11-0033-99

His GraviTrap^™

Cytiva 11-0033-99

• Synonyme(s) : His GraviTrap Column
• Code UNSPSC : 12161501
• Nomenclature NACRES : NA.32

Propriétés

• Conditionnement: pkg of 10 ea
• Fabricant/nom de marque: Cytiva 11-0033-99
• Volume du lit: 1 mL
• D.I. de la colonne: 15 mm
• Matrice: 6% cross-linked agarose
• Taille des particules: 45-165 μm
• Diamètre moyen: 90 μm
• cleaning: 2-14(Ni²⁺-stripped medium)
• working range: 3-12(Ni²⁺-stripped medium.)
• Capacité: ~40 mg binding capacity (histidine-tagged protein/column)(Protein binding capacity is protein-to-protein dependent.); ~40 mg binding capacity(histidine-tagged protein)
• Adéquation: suitable for bioprocess medium

Description

Description générale

His GraviTrap^™ is a prepacked, single-use column for purification of histidine-tagged proteins by immobilized metal affinity chromatography (IMAC). Column gives fast and simple gravity-flow purifications without any need for a purification.

The ready to use column is prepacked with Ni Sepharose^™ 6 Fast Flow, which has a high protein binding capacity, low nickel-ion leakage, and is compatible with denaturing and reducing agents, as well a wide range of additives.

His GraviTrap^™ columns are delivered in a package that can be converted into a column stand to simplify purification. LabMate PD-10 Buffer Reservoir can be connected to the columns for convenient handling of sample volumes above 10 mL.

Caractéristiques et avantages

• For fast and simple purification of histidine-tagged* proteins.
• Convenient, prepacked gravity-flow column.
• Allows direct purification of both clarified and unclarified cell lysates.
• High binding capacity; approximately 40 mg of pure protein per column.
• Compatible with a wide range of reducing agents, detergents, denaturants and other additives.

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informations légales

GraviTrap is a trademark of Cytiva

Sepharose is a trademark of Cytiva

Informations sur la sécurité

• Pictogrammes: GHS09
• Mention d'avertissement: Warning
• Mentions de danger: H226
• Conseils de prudence: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Code de la classe de stockage: 3 - Flammable liquids