E2156
Anti-EGF Receptor antibody, Mouse monoclonal
clone 225, purified from hybridoma cell culture
Synonyme(s) :
Anti-EGFR, Anti-Epidermal Growth Factor Receptor
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About This Item
Source biologique
mouse
Niveau de qualité
Conjugué
unconjugated
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
225, monoclonal
Forme
buffered aqueous solution
Espèces réactives
human
Concentration
~1.5 mg/mL
Technique(s)
immunoprecipitation (IP): 4-8 μg using cell lysate of A431 cells
Isotype
IgG1
Numéro d'accès UniProt
Application(s)
research pathology
Conditions d'expédition
dry ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... EGFR(1956)
Catégories apparentées
Description générale
Monoclonal Anti-EGFR (mouse IgG1 isotype) is derived from the hybridoma 225 produced by the fusion of mouse myeloma cells (NS-1-503 cells) and splenocytes from BALB/c mice immunized with partially purified EGF receptors from A-431 cells. The receptor for epidermal growth factor (EGF) is an integral cell membrane glycoprotein of 170 kDa, which spans the membranes of a wide range of normal and malignant epithelial cells. The EGF receptor has an intracellular domain that exhibits tyrosine kinase activity.
Immunogène
partially purified EGF receptors from human A-431 cells.
Application
Monoclonal Anti-EGF Receptor antibody has been used in antibody-nanoparticle conjugation.
Monoclonal Anti-EGF Receptor antibody is suitable for use in immunoprecipitation (4-8 μg using cell lysate of A431 cells).
Actions biochimiques/physiologiques
EGFR (epidermal growth factor receptors) protein tyrosine kinase is activated when EGF binds the extracellular binding domain. The first detectable response is the autophosphorylation of the C-terminal tyrosine followed by phosphorylation of other endogenous substrates.
EGFR is a tyrosine kinase receptor that regulates cellular several functions such as growth, blood vessel formation, metastasis and invasion. Alterations in EGFR expression have been associated with a wide range of cancers. Thus, drugs that target EGFR signaling have important therapeutic applications in cancer .
Forme physique
0.2 μm filtered solution in 0.01 M phosphate buffered saline, pH 7.4.
Informations légales
This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact The Regents of the University of California.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Code de la classe de stockage
10 - Combustible liquids
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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