KG1a

91030101

• Code UNSPSC : 41106514

Propriétés

• Source biologique: human bone marrow
• Mode de croissance: Suspension
• Caryotype: 2n = 46, pseudodiploid
• Morphologie: Lymphoblast
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human Caucasian bone marrow acute myelogenous leukaemia

Description de la lignée cellulaire

Derived from KG-1, however KG1a is unresponsive to colony stimulating factor in soft agar culture and does not express the Ia-like antigen. KG1a is resistant to phorbol-diester induced macrophage differentiation and cell proliferation. KG1a cells are morphologically, cytochemically and functionally less mature than the parental KG-1. Expresses HLA A30,31; B35; CW4.

Profil d'ADN

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 7
D13S317: 11,12
D16S539: 10,11
D5S818: 13
D7S820: 8,10
THO1: 7,8
TPOX: 7,9
vWA: 14,19

Milieu de culture

IDMEM + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Procédure de repiquage

Maintain cultures between 5x100,000 to 1,000,000 cells/ml. Do not culture below 100,000 cells/ml; 5% CO₂; 37°C.

Autres remarques

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