KARPAS-929

06072606, from human bone marrow

• Synonyme(s) : KARPAS 929, KARPAS, 929, KARPAS929
• Code UNSPSC : 41106514

Propriétés

• Source biologique: human bone marrow
• Mode de croissance: Suspension
• Caryotype: Hyperdiploid with complex aberrant chromosomes
• Morphologie: Round to polygonal cells growing in clusters in suspension
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice

Description

Origine de la lignée cellulaire

Human multiple myeloma

Description de la lignée cellulaire

Established in 1984 from the bone marrow of a 67-year old woman diagnosed with myeloma who survived for one year after diagnosis.

Profil d'ADN

Not specified

Milieu de culture

RPMI 1640 + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS)

Procédure de repiquage

Doubling time approximately 30 hours. Cells grow singly in suspension. Maintain cultures between 5-20 x 10⁵ cells/ml; 5% CO₂; 37 °C. Thaw ampoule and perform a cell count, spin out the DMSO and resuspend in an appropriate volume of media to give a cell concentration of 5 x 10⁵ cells/ml. At subculture perform a cell count and maintain cultures between 5-20 x 10⁵ cells/ml, this tends to equate to a 1:3 split every 2-3 days.

Autres remarques

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Clause de non-responsabilité

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.