C8035

Monoclonal Anti-Chondroitin Sulfate antibody produced in mouse

clone CS-56, ascites fluid

• Synonyme(s) : Chondroitin Sulfate Antibody, Chondroitin Sulfate Antibody - Monoclonal Anti-Chondroitin Sulfate antibody produced in mouse, Cs56 Antibody
• Numéro MDL: MFCD00164568
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Conjugué: unconjugated
• Forme d'anticorps: ascites fluid
• Type de produit anticorps: primary antibodies
• Clone: CS-56, monoclonal
• Espèces réactives: chicken, bovine, mouse, human, pig, rabbit, rat
• Technique(s): indirect immunofluorescence: 1:200 using bovine mammary gland epithelial (BMGE) cells; microarray: suitable
• Isotype: IgM
• Numéro d'accès UniProt: P16112
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... ACAN(176) ; mouse ... Acan(11595) ; rat ... Acan(58968)

Description

Description générale

Chondroitin sulfate is a linear polysaccharide composed of alternating disaccharide glucuronic acid and N-acetylgalactosamine. It contains proteoglycan (PG) of the extracellular matrix (ECM). Monoclonal Anti-Chondroitin Sulfate (mouse IgM isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
This product can be found as purified product that was produced using cell culture hybridoma (SAB4200696)

Spécificité

The antibody has been reported to be specific for the glycosaminoglycan (GAG) portion of native chondroitin sulfate proteoglycan (CSPG). The antibody reacts specifically with chondroitin sulfate types A and C but not with type B (dermatan sulfate), and may be used for localization of chondroitin sulfate in tissues and cultured fibroblasts.

Immunogène

Ventral membranes of chicken gizzard fibroblasts

Application

Monoclonal Anti-Chondroitin Sulfate antibody produced in mouse was used:

• in immunohistochemistry as primary antibody to study of spinal cords and brain cryosections.
• in ELISA and western blot for purification of soluble forms of myelin-associated glycoprotein (MAG) and neural cell adhesion molecule (N-CAM).
• in flow cytometry studies.
• as primary antisera in a study of TGF-β regulation.

Monoclonal Anti-Chondroitin Sulfate can also be used to study chondroitin sulfate proteoglycan (CSPG) distribution and its relationships to specific cell-substrate contacts. It is also suitable for microarray and indirect immunofluorescence at the dilution of 1:200 by using bovine mammary gland epithelial (BMGE) cells.

Actions biochimiques/physiologiques

Chondroitin sulfate is involved in tumor invasion and also plays a vital role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. In Chondroitin sulfate proteoglycan (CSPG), proteoglycan part is involved in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. Chondroitin sulfate is used in osteoarthritis treatment. Upregulation of chondroitin sulfate proteoglycans is observed after neuronal injury. It possess the ability to inhibit axonal regeneration.

Stockage et stabilité

For continuous use, store at 2-8 °C for up to one month.
For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Autres remarques

This product can be found as purified product that was produced using cell culture hybridoma product.
SAB4200696 Anti-Chondroitin Sulfate antibody, Mouse monoclonal
clone CS-56, purified from hybridoma cell culture

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable