Accéder au contenu
MilliporeSigma
Toutes les photos(1)

Documents

A0295

Sigma-Aldrich

Anti-Human IgA (α-chain specific)−Peroxidase antibody produced in goat

affinity isolated antibody

Synonyme(s) :

Goat Anti-Human IgA (α-chain specific)−HRP

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme


About This Item

Numéro MDL:
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Source biologique

goat

Conjugué

peroxidase conjugate

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

secondary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Espèces réactives

human

Concentration

4-11 mg/mL

Technique(s)

direct ELISA: 1:50,000
dot blot: 1:80,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
western blot: suitable

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Vous recherchez des produits similaires ? Visite Guide de comparaison des produits

Description générale

Anti-Human IgA (a-chain specific)-peroxidase antibody is isolated from goat anti-human IgA antiserum by immunospecific purification to remove essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the alpha chain of human IgA.Goat antihuman IgA is conjugated to peroxidase and purified to remove unconjugated material. Specificity of the peroxidase conjugated anti-human IgA is determined by immunoelectrophoresis (IEP) and cross-reactivity of the conjugate is determined by enzyme linked immunoassays (ELISA).

Spécificité

Anti-Human IgA (a-chain specific)-Peroxidase antibody produced in goat conjugate is specific for human IgA when tested against human IgA, IgG, IgM, Bence Jones Kappa and Lambda myeloma proteins. The antibody conjugate does not react with mouse or rat IgG.

Immunogène

Purified human IgA.

Application

Anti-Human IgA (α-chain specific) Peroxidase antibody produced in goat has been used in:
  • Enzyme linked immunosorbent assays (ELISA) at dilution of 1:3,000 and 1: 1,600
  • Tissue anti-transglutaminase antibody (tTG) assay at a dilution of 1:1,000
  • Dot blot: a minimum working dilution of 1:80,000 was determined in a direct chemiluminescence assay using 10ng human IgA/dot. Luminol plus enhancer was used as substrate.
  • Immunohistology using formalin-fixed, paraffin-embedded sections at a dilution of 1:100
  • Transcytosis assay of dimeric IgA (D-IgA)
  • Western Blotting

Actions biochimiques/physiologiques

IgA is a secretory glycoprotein antibody that regulates the physiological balance between commensal intestinal bacteria and the defenses of the host immune system. It is the highest generated antibody isotype. IgAs are mainly secreted in the intestinal mucosa and prevent the commensal microorganisms from invading the intestines.

Forme physique

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT as preservative

Notes préparatoires

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Stockage et stabilité

For continuous use, store at 2-8°C for a maximum of one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Outil de sélection de produits.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Allergic Colitis in Infants Related to Cow
Yu MC, et al.
Pediatrics, 54(1), 49-55 (2013)
Age Dependence and Isotype Specificity of Influenza Virus Hemagglutinin Stalk-Reactive Antibodies in Humans
Nachbagauer R, et al.
mBio, 7(1), 01996-01915 (2016)
An unusual cause of postoperative dyspnoea.
B Cadet et al.
Gut, 53(9), 1278-1278 (2004-08-13)
Ole J B Landsverk et al.
The Journal of experimental medicine, 214(2), 309-317 (2017-01-21)
Plasma cells (PCs) produce antibodies that mediate immunity after infection or vaccination. In contrast to PCs in the bone marrow, PCs in the gut have been considered short lived. In this study, we studied PC dynamics in the human small
Prevalence of celiac disease among blood donors in SAO PAULO - the most populated city in Brazil
Alencar ML, et al.
Clinics, 67(9), 1013-1013 (2012)

Articles

Antibody-based serology tests are useful in identifying subjects with an adaptive immune response to the SARS-CoV-2 virus. Anti-human immunoglobulin antibodies allow for quick and simple, yet reliable assays with easy readouts and can also be adapted for high-throughput screening.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique