37294
Dihydrofolate Reductase from bovine liver
~8 U/mL, ammonium sulfate suspension, off-white
Synonyme(s) :
Tetrahydrofolate Dehydrogenase
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About This Item
Numéro CAS:
Numéro MDL:
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54
Produits recommandés
Source biologique
bovine liver
Niveau de qualité
Forme
ammonium sulfate suspension
Activité spécifique
≥6 U/mg protein
Concentration
~8 U/mL
Couleur
off-white
Densité
1.2 g/mL at 20 °C
Température de stockage
2-8°C
Application
Dihydrofolate reductase (DHFR) is involved in the synthesis of purines, thymidine and glycine in folate metabolism. DHFR, from bovine liver, is used for methotrexate analysis . DHFR is useful to study various cancers such as sarcomas, leukemias and head and neck cancers .
Actions biochimiques/physiologiques
Dihydrofolate reductase (DHFR) reduces dihydrofolic acid to tetrahydrofolic acid, using NADPH as an electron donor. The binding of antitumor agents, such as methotrexate, to DHFR prevents the formation of reduced folates, which are essential for DNA synthesis .
Définition de l'unité
1 U corresponds to the amount of enzyme which converts 1 μmol 7,8-dihydrofolate and NADPH to 5,6,7,8-tetrahydrofolate and NADP per minute at pH 6.5 and 25 °C
Forme physique
Suspension in 3.6 M ammonium sulfate solution, pH 7.0
Autres remarques
In folate metabolism for the synthesis of purines, thymidine and glycine
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves
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Use of dihydrofolate reductase from bovine liver, rather than from L. casei, for determining methotrexate.
R J Brooks
Clinical chemistry, 24(3), 518-519 (1978-03-01)
M A Pesce et al.
Clinical chemistry, 27(3), 380-384 (1981-03-01)
Methotrexate was determined by the homogeneous enzyme immunoassay (EMIT) with the Multistat and CentrifiChem centrifugal analyzers and by the enzyme inhibition assay with use of the Multistat centrifugal analyzer. With both methods, the standard curve extends from 0.2 to 2.0
H B Eastman et al.
Proceedings of the National Academy of Sciences of the United States of America, 88(19), 8572-8576 (1991-10-01)
Dihydrofolate reductase (DHFR; EC 1.5.1.3) is required in folate metabolism for the synthesis of purines, thymidine, and glycine. Although there have been several reports of induction of DHFR enzyme by methotrexate (MTX), a drug that competitively inhibits DHFR, there are
Luca Freschi et al.
Molecular bioSystems, 9(1), 36-43 (2012-10-27)
One of the most important challenges in systems biology is to understand how cells respond to genetic and environmental perturbations. Here we show that the yeast DHFR-PCA, coupled with high-resolution growth profiling (DHFR-qPCA), is a straightforward assay to study the
Inbar Avraham et al.
Proceedings of the National Academy of Sciences of the United States of America, 109(52), E3678-E3686 (2012-12-01)
Plasmodium falciparum causes the deadliest form of human malaria. Its virulence is attributed to its ability to modify the infected RBC and to evade human immune attack through antigenic variation. Antigenic variation is achieved through tight regulation of antigenic switches
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
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