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O-(2,3,4,5,6-Pentafluorobenzyl)hydroxylamine hydrochloride

for GC derivatization, LiChropur™, ≥99.0% (AT)

Synonyme(s) :

PFBHA·HCl

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About This Item

Formule linéaire :
C6F5CH2ONH2·HCl
Numéro CAS:
57981-02-9
Poids moléculaire :
249.57
Numéro Beilstein :
4031190
Numéro CE :
261-057-7
Numéro MDL:
MFCD00012953
Code UNSPSC :
12000000
ID de substance PubChem :
329767306
Nomenclature NACRES :
NA.22

Qualité

for GC derivatization
LiChropur™

Niveau de qualité

100

Pureté

≥99.0% (AT)

Forme

solid

Pertinence de la réaction

reagent type: derivatization reagent
reaction type: Alkylations

Technique(s)

gas chromatography (GC): suitable

Pf

212-218 °C
227 °C (subl.) (lit.)

Solubilité

H2O: 5%, clear

Chaîne SMILES 

Cl.NOCc1c(F)c(F)c(F)c(F)c1F

InChI

1S/C7H4F5NO.ClH/c8-3-2(1-14-13)4(9)6(11)7(12)5(3)10;/h1,13H2;1H

Clé InChI

HVMVKNXIMUCYJA-UHFFFAOYSA-N

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Description générale

O-(2,3,4,5,6-Pentafluorobenzyl)hydroxylamine hydrochloride (PFBHA.HCl) is a derivatizing agent.

Application

PFBHA.HCl has been used for derivatization of metabolites with carbonyl groups followed by analysis via GC-MS. It has also been used in aldehyde derivatization followed by aldehydic hydrolysis using GC/MS.

Conditionnement

Bottomless glass bottle. Contents are inside inserted fused cone.

Autres remarques

Sensitive derivatizing agent for electron capture gas chromatographic analysis of carbonyl-containing compounds: keto steroids and carbohydrates

Informations légales

LiChropur is a trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

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Certificats d'analyse (COA)

Lot/Batch Number
BCCG5577
BCCF8143
BCCD9493
BCCD2221
BCCB4662

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Plasmalogen degradation by oxidative stress: production and disappearance of specific fatty aldehydes and fatty alpha-hydroxyaldehydes.
Stadelmann-Ingrand S
Free Radical Biology & Medicine, 31(10), 1263-1271 (2001)
Degradation of fluoranthene by Pasteurella sp. IFA and Mycobacterium sp. PYR-1:isolation and identification of metabolites.
Sepic E, Bricelj M, Leskovsek H.
Journal of Applied Microbiology, 85(4), 746-754 null
Birgit Schulze et al.
Analytical biochemistry, 348(2), 269-283 (2005-11-26)
A GC-MS-based method for the simultaneous quantification of common oxylipins along with labile and highly reactive compounds based on in situ derivatization with pentafluorobenzyl hydroxylamine to the corresponding O-2,3,4,5,6-pentafluorobenzyl oximes (PFB oximes) is presented. The approach covers oxo derivatives such
Chunhui Deng et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 810(2), 269-275 (2004-09-24)
Analysis of breath acetone has been used as a diagnostic tool for diabetes. Due to its nature of volatility and activity, it is very difficult to accurately measure the concentration of acetone in human breath by gas chromatography-mass spectrometry (GC-MS).
Yun-Gon Kim et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 893-894, 177-181 (2012-03-31)
Nucleotide diphosphate (NDP) sugars are widely present in antibiotics and glycoconjugates, such as protein- and lipid-linked oligosaccharides, where they act as substrates for glycosyltransferase in eukaryotes and prokaryotes. Among NDP sugars, NDP-4-keto sugars are key intermediates in the synthesis of
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