10109339001

Sorbitol Dehydrogenase (SDH)

from sheep liver

• Synonyme(s) : SDH, Sorbitol Dehydrogenase, sorbitol
• Numéro de classification (Commission des enzymes): 1.1.1.14 (BRENDA, IUBMB)
• Code UNSPSC : 12352204

Propriétés

• Source biologique: sheep liver
• Niveau de qualité: 100
• Forme: lyophilized
• Activité spécifique: ~40 units/mg protein (At 25 °C with D-fructose as the substrate.)
• Poids mol.: 115 kDa
• Conditionnement: pkg of 10 mg (60 mg lyophilizate)
• Fabricant/nom de marque: Roche
• Concentration: 0.16 mg/mL Biuret
• Technique(s): activity assay: suitable
• Couleur: white
• pH optimal: 7.5
• Solubilité: water: 60 mg/mL, clear, colorless
• Adéquation: suitable for ELISA applications
• Numéro d'accès NCBI: XP_004010700
• Numéro d'accès UniProt: P07846
• Application(s): life science and biopharma
• Activité étrangère: ADH <0.00182%; GIDH <0.00152%; Glucose-DH <0.00182% (NAD as coenz.); LDH <0.00167%; MDH <0.00198%; NADH oxidase 0.00532%
• Température de stockage: 2-8°C
• Informations sur le gène: sheep ... SORD(101115280)

Description

Description générale

L-iditol:NAD⁺ 5′-oxidoreductase
Sorbitol dehydrogenase (SDH) from sheep liver has a molar mass of 152kDa. The protein is a tetramer of four identical subunits. Each of these subunits has 355 amino acid residues, of which 10 are cysteine residues. Each subunit contains a zinc atom at the active site, which is associated with three protein ligands and a water molecule. The zinc atom associates with the oxygen of the sorbitol hydroxyl or of the fructose carbonyl interconverted during catalysis.

Application

Reduces L-iditol to L-sorbose. Also acts on D-glucitol and other closely related sugar alcohols. Allows the reduction of ketones to polyols (see aldolases for the synthesis of ketoses).

Actions biochimiques/physiologiques

Sorbitol dehydrogenase (SDH) catalyzes the reversible NAD-linked conversion of D-sorbitol to D-fructose and forms a part of the sorbitol pathway, which is a crucial bypass to glycolysis for glucose metabolism. This pathway has been found to be associated with the accumulation of sorbitol in lens, which results in diabetic cataractogenesis. SDH also oxidizes various polyols and other secondary alcohols into their corresponding ketones.

Qualité

Contaminants: <0.01% ADH, <0.02% GIDH and glucose dehydrogenase, each, <0.05% LDH and MDH, each.

Définition de l'unité

One unit (U) sorbitol dehydrogenase will reduce 1 mol of D-fructose in one minute at +25 °C and pH 7.6 [triethanolamine buffer; 150 mM fructose (nonsaturating concentration)]. The above assay consumes 1 mol of NADH per mol of D-sorbitol formed.

Forme physique

Lyophilizate (60 mg contain 10 mg enzyme protein and 50 mg maltose).

Notes préparatoires

Activator: The reactions (oxidation or reduction) are fastest in Tris or triethanolamine buffer.
Stabilizers: Maltose is used as stabilizer.
Storage conditions (working solution): An aqueous solution is stable at 2 to 8 °C for several weeks.

Stockage et stabilité

Store at 2 to 8 °C. (Store the lyophilizate dry.)

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): does not flash
• Point d'éclair (°C): does not flash