ABE217

Anti-CUX1 Antibody, a.a. 861

from rabbit, purified by affinity chromatography

• Synonyme(s) : Homeobox protein cut-like 1, CCAAT displacement protein, CDP, Homeobox protein cux-1
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: human
• Technique(s): immunoprecipitation (IP): suitable; western blot: suitable
• Numéro d'accès NCBI: NP_853530
• Numéro d'accès UniProt: P39880
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... CUX1(1523)

Description

Description générale

Homeobox protein cut-like 1 (CUX1) is also known as CCAAT displacement protein (CDP) and Homeobox protein cux-1. CUX1 is thought to repress expression of developmentally regulated genes involved in mammalian development, likely through the prevention of CCAAT factor binding at promoters. CUX1 also represses T-cell receptor (TCR) beta enhancer function by binding to matrix attachment region beta (MARbeta), and is a component of the nf-munr repressor, which binds to matrix attachment regions (MARs) of the immunoglobulin heavy chain enhancer.

Spécificité

Other homologies: Mouse (95% sequence homology).

This antibody recognizes the region of amino acids beginning at 861 of CUX1. This antibody is expected to cross react with all known isoforms of CUX1.

Immunogène

Epitope: Region of amino acids beginning at 861

GST-tagged recombinant protein corresponding to a region of amino acids beginning at 861 of human CUX1.

Application

Detect Cux1 using this rabbit polyclonal antibody, Anti-CUX1 Antibody, a.a. 861 validated for use in western blotting & IP.

Immunoprecipitation Analysis: 1.25 µg from a representative lot immunoprecipitated CUX1 in 500 µg of HeLa nuclear extract.

Alexa Fluor^™ is a registered trademark of Life Technologies.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Qualité

Evaluated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: 0.25 µg/mL from a representative lot detected CUX1 in 10 µg of HeLa nuclear extract.

Description de la cible

~200 kDa and ~110 kDa observed. Uniprot describes 11 different isoforms. A full length protein has been described at 200 kDa and is proteolytically cleaved into the p150, p110, p90, and p80 isoforms. Additionally, the p75 isoform is the result of alternative splicing (Sansregret, L, et al. (2008). Gene. 412(1-2):84-94.). Uncharacterized band(s) may be observed in some cell lysates.

Forme physique

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
HeLa nuclear extract

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

ALEXA FLUOR is a trademark of Life Technologies

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable