480010-M

NDSB-256

A non-detergent sulfobetaine. Reported to prevent protein aggregation and facilitate the renaturation of chemically and thermally denatured proteins.

• Synonyme(s) : NDSB-256, Dimethylbenzylammonium Propane Sulfonate
• Formule empirique (notation de Hill): C₁₂H₁₉NO₃S
• Numéro CAS: 81239-45-4
• Poids moléculaire : 257.35
• Numéro MDL: MFCD00225018
• Code UNSPSC : 12352200

Propriétés

• Niveau de qualité: 100
• Pureté: ≥99% (TLC)
• Forme: solid
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; desiccated (hygroscopic)
• Couleur: white
• Solubilité: water: 200 mg/mL
• Température de stockage: 10-30°C
• InChI: 1S/C12H19NO3S/c1-13(2,9-6-10-17(14,15)16)11-12-7-4-3-5-8-12/h3-5,7-8H,6,9-11H2,1-2H3
• Clé InChI: MEJASPJNLSQOAG-UHFFFAOYSA-N

Description

Description générale

A member of the class of nondetergent sulfobetaines. Unlike CHAPS and other zwittergents, NDSBs are nondenaturing even at 1 M concentrations. NDSBs have been used to improve the efficiency of solubilization of membrane proteins, to isolate nuclear proteins, to chromatographically purify halophilic proteins in low ionic strength buffers, and to crystallize proteins. They are zwitterionic over a wide pH range and do not absorb significantly in the near UV range. Reported to prevent protein aggregation and facilitate the renaturation of chemically and thermally denatured proteins. NDSBs can be easily removed by dialysis. At 1 M, NDSB-256 will restore 30% of the enzymatic activity of denatured egg white lysozyme; at 800 mM it will restore 16% of the enzymatic activity of denatured β-galactosidase.

A non-detergent sulfobetaine. Reported to prevent protein aggregation and facilitate the renaturation of chemically and thermally denatured proteins. Zwitterionic over a wide pH range. Easily removed by dialysis. At 1 M, NDSB-256 will restore 30% of the enzymatic activity of denatured egg white lysozyme; at 800 mM it will restore 16% of the enzymatic activity of denatured β-galactosidase.

Avertissement

Toxicity: Irritant (B)

Reconstitution

Following reconstitution, store at room temperature (15-30°C). Stock solutions are stable for up to 3 months at room temperature.

Autres remarques

Goldberg, M.E., et al. 1996. Folding & Design1, 21.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable