375228

Hepatocyte Growth Factor, Human, Recombinant, S. frugiperda

• Synonyme(s) : rhHGF
• Code UNSPSC : 12352202

Propriétés

• Pureté: ≥95% (SDS-PAGE)
• Niveau de qualité: 100
• Forme: lyophilized
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze
• Impuretés: ≤1 EU/μg endotoxin (EU/μg HGF)
• Conditions d'expédition: ambient
• Température de stockage: −70°C

Description

Description générale

Recombinant, human hepatocyte growth factor expressed in S. frugiperda insect cells. Pleiotropic growth factor that has also been called scatter factor, hepatopoietin A, and mammary growth factor. Through binding to its high affinity met receptor, HGF has marked and varied effects on hepatocytes and other epithelial and endothelial cell types. HGF-induced proliferation of primary hepatocytes is mediated by activation of PI 3-kinase. Acts as a potent mitogen for mature hepatocytes.

Recombinant, human hepatocyte growth factor expressed in Spodoptera frugiperda insect cells. Pleiotropic growth factor that has also been called scatter factor, hepatopoietin A, and mammary growth factor. Through binding to its high affinity met receptor, HGF has marked and varied effects on hepatocytes and other epithelial and endothelial cell types. HGF-induced proliferation of primary hepatocytes is mediated by activation of PI 3-kinase. Acts as a potent mitogen for mature hepatocytes.

Actions biochimiques/physiologiques

ED₅₀ = 20-40 ng/ml as measured by its ability to stimulate ³H-thymidine incorporation in an HGF-responsive epithelial cell line, 4MBr-5

Avertissement

Toxicity: Standard Handling (A)

Forme physique

Lyophilized from a sterile-filtered solution in 20 mM Sodium Phosphate, 0.5 M NaCl, 50 µg BSA/µg HGF, pH 7.0.

Reconstitution

Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for 1 month at 4°C or for up to 3 months at -70°C.

Reconstitute to a concentration of ≥1 µg/ml in sterile PBS containing ≥0.1% HSA or BSA.

Autres remarques

Kaido, T., et al. 1996. Biochem. Biophys. Res. Commun. 218, 1.
Kobayashi, Y., et al. 1996. Biochem. Biophys. Res. Commun. 220, 7.
Skouteris, G.G., et al. 1996. Biochem. Biophys. Res. Commun. 218, 229.
Rubin, J.S., et al. 1993. Biochim. Biophys. Acta 1155, 357.
Strain, A.J. 1993. J. Endocrinol. 137, 1. Nakamura, T., et al. 1989. Nature 342, 440.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3