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Chromolith® WP 300 Protein A (monolithic) HPLC Columns

L × I.D. 25 mm x 4.6 mm HPLC Column

Synonyme(s) :

Chromolith® WP 300 Protein A 25-4.6 HPLC column

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About This Item

Code UNSPSC :
41115700
Nomenclature NACRES :
SB.52

product name

Chromolith® WP 300 Protein A, L × I.D. 25 mm x 4.6 mm HPLC column

Matériaux

PEEK column

Gamme de produits

Chromolith®

Caractéristiques

endcapped: no
endcapped: no

Conditionnement

1 ea of

Paramètres

2 - 45 °C temp. range
2-45 °C temp. range
200 bar pressure
200 bar pressure

Technique(s)

HPLC: suitable
HPLC: suitable
LC/MS: suitable

Colonne, L × D.I.

2.5 cm × 4.6 mm

Superficie

120 m2/g

Matrice

monolithic silica
monolithic silica

Groupe de la matrice active

Protein A bonding phase
Protein A bonding phase

Dimension de pores

2 μm macroporosity
2 μm macroporosity
30 nm mesoporosity
30 nm mesoporosity

operating pH range

1.5-7.5

pH de fonctionnement

1.5-7.5

Technique de séparation

affinity

Température de stockage

2-8°C
2-8°C

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Description générale

Affinity chromatography is a selective technique which takes advantage of specific molecular interactions, for example antigen and antibody. The Chromolith® WP 300 Protein A HPLC column is designed to monitor monoclonal antibody titer and yield determination from cell-culture supernatants. This analytical scale procedure helps to optimize the titer of monoclonal antibody for the optimal time for harvest of the monoclonal antibody products.

Chromolith® WP 300 Protein A 25-4.6 columns are made of monolithic silica with a characteristic bimodal pore structure.

Key Benefits:
—Rapid and accurate titer determination across a wide range of injection volumes
—Consistent binding efficiencies even at high flow rates
—Expanded column lifetimes

Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns

Chromolith® WP 300 Protein A is available in 2 mm I.D. providing improved efficiency, sensitivity, and enhanced MS-compatibility.
Flyer: Rapid and Robust Antibody Quantitation - Chromolith® WP 300 Protein A HPLC columns

All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument

Informations légales

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificats d'analyse (COA)

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Articles

Fast Analysis of Monoclonal Antibodies Using Silica Monoliths Designed for Bioanalysis

Silica monoliths with larger mesopores are a powerful tool for the fast separation of biomolecules including mAbs. The immobilization of protein A on epoxy-modified widepore (WP) silica rods enabled highly reproducible and fast, one minute, separations of mAbs. The Chromolith® WP 300 is also available with a protein A phase.

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Analytical Chromolith® HPLC columns for Bio applications, 300A mesopores: RP-18, RP-8, RP-4, Prot-A, Epoxy

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