07-1224

Anti-PP6C Antibody

from rabbit

• Synonyme(s) : H.sapiens mRNA for protein phosphatase 6, Serine/threonine-protein phosphatase 6, Protein phosphatase 6, catalytic subunit, Serine/threonine protein phosphatase catalytic subunit
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Espèces réactives: mouse, rat, bovine, human
• Technique(s): immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• Isotype: IgG
• Numéro d'accès NCBI: NM_002721.3
• Numéro d'accès UniProt: O00743
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... PPP6C(5537)

Description

Description générale

This gene encodes the catalytic subunit of protein phosphatase, a component of a signaling pathway regulating cell cycle progression.

Spécificité

Recognizes the catalytic subunit of Protein Phosphatase 6, MW ~33kDa.

Immunogène

The immunogen used to the purified peptide conjugated to KLH corresponding to the sequence NH2-Cys-Ala-Val-Pro-Asp-Ser-Glu-Arg-Val-Ile- Pro-Pro-Arg-Thr-Thr-Thr-Pro-Tyr-COOH.

Application

Immunoprecipitation: A previous lot of this antibody was used in immunoprecipitation at a dilution of 10-20 µg/mL.
Immunohistochemistry(paraffin):
Optimal Staining of PP6 Polyclonal Antibody:
Human Testis
PP6 staining of round spermatids in normal human
testis, tissue pretreated with citrate buffer, pH 6.0.
Antibody was diluted 1:100, using IHC-Select
detection with HRP-DAB.

Detect PP6C using this Anti-PP6C Antibody validated for use in WB, IP, IH(P).

Research Category
Signaling

Research Sub Category
Kinases & Phosphatases

Qualité

Routinely evaluated by Western Blot on Jurkat lysates.

Western Blot Analysis: 1:500 dilution of this lot detected PP6C on 10 μg of Jurkat lysates.

Description de la cible

Recognizes the catalytic subunit of Protein Phosphatase 6 (PP6), MW ~33kDa

Liaison

Replaces: AB4091

Forme physique

Format: Purified

Purified rabbit polyclonal IgG in buffer containing PBS with 0.08% sodium azide.

Stockage et stabilité

Maintain at -20°C in undiluted aliquots for up to 1 year from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Remarque sur l'analyse

Control
Jurkat Cell Lysate

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable