Method development starts with the sample and its analytes. Depending on the sample and the separation goals the selection of the column chemistry, column physics and the best suitable mobile phase conditions for the specific analytes and the individual needs is a key aspect for successful method development. In addition, time and productivity becomes more and more important. This fact has a very high influence on method development. It is crucial to avoid trouble and find the most cost-efficient method to get precise and sensitive results.
This seminar gives an overview about the available column base materials for HPLC and UHPLC, their benefits and limitations and the column chemistries for separation across compound polarities.
In this webinar, you will learn:
- Understand the challenges in method development and how to overcome them (selection of stationary phase and mobile phase)
- Learn how to leverage lab efficiency by simplifying the HPLC workflows and getting faster results
- Learn how to prevent potential trouble with improved system set-up
Speakers
Petra Lewits
MilliporeSigma
Global Product Manager for Analytical Chromatography, HPLC columns
Petra has worked with chromatography for 40 years. She started her career in separation science in 1983 using several chromatographic methods, including HPLC, TLC, GC and solid-phase extraction (SPE), for food control in a governmental institution lab. In 1986 she joined the pharmaceutical R&D and QC department (Merck KGaA, Darmstadt, Germany) where she developed HPLC methods for R&D and QC of pharmaceutical APIs and formulations as well as for diet convenience foods. From 1991 on she supported customers in different positions with technical and application-related inquires in Europe as a sales specialist for HPLC instruments, also providing technical support for chromatography and complaint management. Since 2002 she has been a Global Product Manager for Analytical Chromatography (HPLC). In 2014 she began focusing on thin-layer chromatography (TLC). Since April 2018 Petra has had global responsibility for analytical HPLC columns.
Analytical chemistry
- Low pressure liquid chromatography
Durée:1h 20min
Langue:English
Session 1:présenté(e) November 7, 2023
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