What Does it Cover?
Duolink® proximity ligation assay (PLA) technology allows you to visualize protein interactions with cellular localization and quantities by amplifying signals corresponding to single and post-translational protein events. With 1000x sensitivity and high specificity, this protein detection technology allows to visualize protein functions, all within a native cell. The PLA method provides:
- Visual protein interactions
− Both stable and transient - Endogenous protein detection
− No overexpression or genetic manipulation - High specificity
− Use of two antibodies/probes eliminates false positives - Single molecule sensitivity
− Rolling circle amplification makes proteins visible - No special equipment needed
− Standard immunofluorescence methods
This webinar will review how to work with PLA technology and provide an overview of their potential along with example applications.
What Will You Learn?
- How to work with Duolink® PLA technology
- Overview of most relevant applications using Duolink®
- Product offering to run an assay with PLA using Immunofluorescence or Flow Cytometry
- Benefits of working with highly specific and powerful technology to study protein biology
Who Should Attend?
Researchers in the T-Cell, adaptive immunotherapy, and immuno-oncology fields
Speakers
Holly Johnston, Ph.D.
MilliporeSigma
Product Manager - Duolink®
Cláudia Emanuele
MilliporeSigma
Field Marketing Specialist
Protein biology
- Flow cytometry
Durée:52min
Langue:English
Session 1:présenté(e) May 19, 2021
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