{"metaDataObj":{"isNoIndex":false,"isNoFollow":false,"keywords":["Protocol","Large molecule HPLC"],"title":"HPLC Analysis of the Monoclonal Antibody (mAb) Erbitux (Cetuximab) on BIOshell A400 Protein C4","seoSchemas":[{"@context":"https://schema.org","@type":"BreadcrumbList","itemListElement":[{"@type":"ListItem","position":1,"name":"Large Molecule HPLC","item":"%%HOMEPAGE_URL%%/CA/en/applications/analytical-chemistry/liquid-chromatography/large-molecule-hplc"},{"@type":"ListItem","position":2,"name":"HPLC Analysis of the Monoclonal Antibody (mAb) Erbitux (Cetuximab) on BIOshell^™ A400 Protein C4"}]}],"description":"HPLC Analysis of the Monoclonal Antibody (mAb) Erbitux (Cetuximab) on BIOshell™ A400 Protein C4","pageType":"Protocol","ogImageMetaInfo":[{"ogTitle":"HPLC Analysis of the Monoclonal Antibody (mAb) Erbitux (Cetuximab) on BIOshell A400 Protein C4","ogType":"website","ogImage":"https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/social-media/vibrant-m/vibrant-m.jpg","ogUrl":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux","ogDescription":"HPLC Analysis of the Monoclonal Antibody (mAb) Erbitux (Cetuximab) on BIOshell™ A400 Protein C4"}],"translationMethod":null,"canonicalUrl":null},"designPath":"/libs/settings/wcm/designs/default","cssClassNames":"page basicpage","title":"HPLC Analysis of the Monoclonal Antibody (mAb) Erbitux (Cetuximab) on BIOshell A400 Protein C4","lastModifiedDate":1601320349144,"templateName":"technical-article---protocol-page-template","language":"en",":items":{"root":{"columnClassNames":{"rightrailresponsivecontainer":"aem-GridColumn aem-GridColumn--default--3 aem-GridColumn--offset--default--0 aem-GridColumn--default--none","footer":"aem-GridColumn aem-GridColumn--default--12","utilityresponsivecontainer":"aem-GridColumn aem-GridColumn--default--3 aem-GridColumn--offset--default--0 aem-GridColumn--default--none","bodyresponsivecontainer":"aem-GridColumn aem-GridColumn--default--9 aem-GridColumn--offset--default--0 aem-GridColumn--default--none","topresponsivecontainer":"aem-GridColumn aem-GridColumn--default--9 aem-GridColumn--offset--default--0 aem-GridColumn--default--none"},"gridClassNames":"aem-Grid aem-Grid--12 aem-Grid--default--12","columnCount":12,"allowedComponents":{"applicable":false,"components":[{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/aboutus","title":"About Us Text"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/accordion","title":"Accordion"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/audio","title":"Audio"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/author","title":"Author"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/breadcrumb","title":"Breadcrumbs - Page Path"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/button","title":"Button"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/card","title":"Card"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/carousel","title":"Carousel"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/categorysearchresults","title":"Category Search Results"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/cftext","title":"CF Text"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/contact","title":"Contact Component"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/container","title":"Container component"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/dealerlicense","title":"Dealer License"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/dynamicproducttable","title":"Dynamic Product Table"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/event-sidebar","title":"Event Sidebar"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/eventlisting","title":"Event Listing"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/experiencefragment","title":"Experience Fragment"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/featuretiles","title":"Feature Tiles"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/footer","title":"Footer"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/formAssembly","title":"Form Assembly"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/gatedbutton","title":"Gated Button"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/gatedcontent","title":"Gated Content"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/globalfooter","title":"Global Footer"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/header","title":"Header"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/herocomponent","title":"Hero Component"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/workflows/horizontal","title":"Workflow Horizontal"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/horizontaltab","title":"Tabbed Horizontal Navigation"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/image","title":"Image"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/leftnavigation","title":"Navigation Model"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/locationmap","title":"Map"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/pressreleasegrid","title":"Related Press releases"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/pressreleaselisting","title":"Press release listing"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/presstext","title":"Region Text"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/primarycontent","title":"Primary Content"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/printsocialsharing","title":"Print Social sharing"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/productRecommendationCarousel","title":"Product Recommendation Carousel"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/productselector","title":"Product Selector Tool"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/promobanner","title":"Promo Banner"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/rawhtml","title":"Raw HTML"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/recommendedSearch","title":"Recommended Search Term"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/reference","title":"Reference"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/relatedContentCarousel","title":"Related Content Carousel"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/relatedcontentgrid","title":"Related Content Grid"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/relatedcontentlinks","title":"Related Content Links"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/relatedproductresources","title":"Related Product Resources"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/relatedproducts","title":"Related Products Carousel"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/relatedproductsrightrail","title":"Related Products Right Rail"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/relatedwebinar","title":"Related Webinar"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/sectiondivider","title":"Section Divider"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/selfServiceReviewForm","title":"Self Service Review Form"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/shopProductRightRail","title":"Shop Product Right Rail"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/sitecontentsearch","title":"Site Content Search"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/sitemap","title":"Site Map"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/speakerpage","title":"Speaker Page"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/speakers-list","title":"Speakers List"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/table","title":"Static Table"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/tagbasedbreadcrumb","title":"Breadcrumbs - Tag Based"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/targetcontainer","title":"Target Container component"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/targetescapehatch","title":"Target Escape Hatch"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/text","title":"Text"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/textbanner","title":"Text Banner"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/title","title":"Title"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/topPagesCarousel","title":"Top Pages Carousel"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/topnavigation","title":"Top Navigation"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/upcomingeventsgrid","title":"Upcoming Events"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/userEngageCarousel","title":"User Engage Carousel"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/workflows/vertical","title":"Workflow Vertical"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/video","title":"Video"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/webinarlisting","title":"Webinar listing"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/webinarsidebar","title":"Webinar Sidebar"},{"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/cms-commons/components/content/worldwideofficelisting","title":"Worldwide Office Listing"}]},":items":{"topresponsivecontainer":{"columnClassNames":{"tagbasedbreadcrumb":"aem-GridColumn aem-GridColumn--default--9","cq:responsive":"aem-GridColumn aem-GridColumn--default--9","title":"aem-GridColumn aem-GridColumn--default--9"},"cssClassName":"","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--default--9","columns":12,"hasFeatureCard":false,"halfGatedContent":false,"items":[{"url":null,"title":null,"description":null,"lastModified":1601320348962,"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/topresponsivecontainer/tagbasedbreadcrumb","name":"tagbasedbreadcrumb"},{"url":null,"title":null,"description":null,"lastModified":1601320348968,"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/topresponsivecontainer/title","name":"title"}],"allowedComponents":{"applicable":false,"components":[]},"authenticatedUserBGColor":"","mobileSpacing":{},"unauthenticatedUserBGColor":"",":items":{"tagbasedbreadcrumb":{"items":[{"url":"/CA/en","title":"Home","titleEng":"Home","active":false,"nonClickable":false},{"url":"/CA/en/applications/analytical-chemistry/liquid-chromatography/large-molecule-hplc","title":"Large Molecule HPLC","titleEng":"Large Molecule HPLC","active":false,"nonClickable":false},{"url":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux","title":"HPLC Analysis of the Monoclonal Antibody (mAb) Erbitux (Cetuximab) on BIOshell^™ A400 Protein C4","titleEng":"HPLC Analysis of the Monoclonal Antibody (mAb) Erbitux (Cetuximab) on BIOshell^™ A400 Protein C4","active":true,"nonClickable":true}],":type":"cms-commons/components/content/tagbasedbreadcrumb"},"title":{"anchorId":null,"openNewTab":false,"linkURL":null,"text":"HPLC Analysis of the Monoclonal Antibody (mAb) Erbitux (Cetuximab) on BIOshell^™ A400 Protein C4","type":"h1",":type":"cms-commons/components/content/title"}},":itemsOrder":["tagbasedbreadcrumb","title"],"desktopSpacing":{},":type":"cms-commons/components/content/container"},"utilityresponsivecontainer":{"columnClassNames":{"cq:responsive":"aem-GridColumn aem-GridColumn--default--3"},"cssClassName":"cms-container--top","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--default--3","columns":12,"hasFeatureCard":false,"halfGatedContent":false,"items":[],"allowedComponents":{"applicable":false,"components":[]},"authenticatedUserBGColor":"","mobileSpacing":{},"unauthenticatedUserBGColor":"",":items":{},":itemsOrder":[],"desktopSpacing":{},":type":"cms-commons/components/content/container"},"bodyresponsivecontainer":{"columnClassNames":{"reference":"aem-GridColumn aem-GridColumn--default--9","bottomcontainer":"aem-GridColumn aem-GridColumn--default--9","dynamicproducttable":"aem-GridColumn aem-GridColumn--default--9","middleresponsivecontainer":"aem-GridColumn aem-GridColumn--default--9","topcontainer":"aem-GridColumn aem-GridColumn--default--9","cq:responsive":"aem-GridColumn aem-GridColumn--default--9"},"cssClassName":"","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--default--9","columns":12,"hasFeatureCard":false,"halfGatedContent":false,"items":[{"url":null,"title":null,"description":null,"lastModified":1601320349043,"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/bodyresponsivecontainer/topcontainer","name":"topcontainer"},{"url":null,"title":null,"description":null,"lastModified":1601320349099,"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/bodyresponsivecontainer/dynamicproducttable","name":"dynamicproducttable"},{"url":null,"title":null,"description":null,"lastModified":1601320349105,"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/bodyresponsivecontainer/middleresponsivecontainer","name":"middleresponsivecontainer"},{"url":null,"title":null,"description":null,"lastModified":1601320349109,"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/bodyresponsivecontainer/reference","name":"reference"},{"url":null,"title":null,"description":null,"lastModified":1601320349114,"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/bodyresponsivecontainer/bottomcontainer","name":"bottomcontainer"}],"allowedComponents":{"applicable":false,"components":[]},"authenticatedUserBGColor":"","mobileSpacing":{},"unauthenticatedUserBGColor":"",":items":{"topcontainer":{"columnClassNames":{"image":"aem-GridColumn aem-GridColumn--default--7 aem-GridColumn--offset--default--0 aem-GridColumn--default--none","table_2114408878":"aem-GridColumn aem-GridColumn--default--12","table":"aem-GridColumn aem-GridColumn--default--12"},"cssClassName":"","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--12 aem-Grid--default--12","columns":12,"hasFeatureCard":false,"halfGatedContent":false,"items":[{"url":null,"title":null,"description":null,"lastModified":1601320349067,"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/bodyresponsivecontainer/topcontainer/image","name":"image"},{"url":null,"title":null,"description":null,"lastModified":1601320349085,"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/bodyresponsivecontainer/topcontainer/table","name":"table"},{"url":null,"title":null,"description":null,"lastModified":1601320349092,"path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux/jcr:content/root/bodyresponsivecontainer/topcontainer/table_2114408878","name":"table_2114408878"}],"allowedComponents":{"applicable":false,"components":[]},"authenticatedUserBGColor":"","mobileSpacing":{},"unauthenticatedUserBGColor":"",":items":{"image":{"anchorId":null,"src":"/deepweb/assets/sigmaaldrich/marketing/global/images/technical-documents/protocols/analytical-chemistry/large-molecule-hplc/g006254-large/g006254-large.jpg","alt":"HPLC Analysis of the Monoclonal Antibody (mAb) Erbitux (Cetuximab) on BIOshell™ A400 Protein C4","externalUrl":false,"title":"HPLC Analysis of the Monoclonal Antibody (mAb) Erbitux (Cetuximab) on BIOshell™ A400 Protein C4","uuid":"8c8565c8-db7e-412a-8fb9-12bd8f2ea5b5","widths":[],"link":"","caption":"","displayPopupTitle":true,":type":"cms-commons/components/content/image"},"table":{"anchorId":null,"enableSort":false,"verticalTable":false,"headline":"Conditions","title":null,"caption":null,"table":"\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n

Column	BIOshell™ A400 Protein C4, 10 cm X 2.1 mm I.D., 3.4 µm particles (66825-U)
Column temp.	90 °C
Mobile phase	(A) 80:20, 0.1% TFA in water: 0.1% TFA in acetonitrile; (B) 20:80, 0.1% TFA in water: 0.1% TFA in acetonitrile
Gradient	20 to 35%B in 10 min (or 5 min)
Flow rate	0.2 mL/min
Pressure	470 psi (or 940 psi)
Sample	1 g/L mAb in 20 mM Tricine/KOH, pH 7
Injection	0.4 µL
Detector	UV, 215 nm

\n",":type":"cms-commons/components/content/table"},"table_2114408878":{"anchorId":null,"enableSort":false,"verticalTable":false,"headline":"Description","title":null,"caption":null,"table":"\n\n\n\n\n\n\n\n\n\n\n

Analysis Note	Antibodies can exhibit considerable hydrophobicity, and so efficient reversed-phase elution of this intact mAb requires the use of high temperature. The top chromatogram illustrates that BIOshell^™ Fused-Core^® columns can be operated at high flow rate while maintaining good peak shape at a reasonable back pressure.
Categories	Analytical Chromatography, Proteins
Featured Industry	Life Science and Biopharma
Legal Information	BIOshell is a trademark of Sigma-Aldrich Co. LLC, Fused-Core is a registered trademark of Advanced Materials Technology, Inc.
Suitability	Application for HPLC

\n",":type":"cms-commons/components/content/table"}},":itemsOrder":["image","table","table_2114408878"],"desktopSpacing":{},":type":"cms-commons/components/content/container"},"dynamicproducttable":{"anchorId":null,"productsArray":["5.33001 Supelco (MM)","34851 Sigma-Aldrich (SIGALD)","1.00029 Supelco (MM)","73594 Supelco (SIAL)","70221 Supelco (SIAL)","66830-U Supelco (SUPELCO)","66825-U Supelco (SUPELCO)","1.11727 Supelco (MM)","5.33002 Supelco (MM)","1.06007 Supelco (MM)","1.06035 Supelco (MM)","5.43841 Supelco (MM)","69372 Millipore (SIAL)","302031 Sigma-Aldrich (SIGALD)","1.15333 Supelco (MM)"],"isShowImage":true,"maxRows":20,"products":[{"productKey":"533001","brandKey":"MM"},{"productKey":"34851","brandKey":"SIGALD"},{"productKey":"100029","brandKey":"MM"},{"productKey":"73594","brandKey":"SIAL"},{"productKey":"70221","brandKey":"SIAL"},{"productKey":"66830U","brandKey":"SUPELCO"},{"productKey":"66825U","brandKey":"SUPELCO"},{"productKey":"111727","brandKey":"MM"},{"productKey":"533002","brandKey":"MM"},{"productKey":"106007","brandKey":"MM"},{"productKey":"106035","brandKey":"MM"},{"productKey":"543841","brandKey":"MM"},{"productKey":"69372","brandKey":"SIAL"},{"productKey":"302031","brandKey":"SIGALD"},{"productKey":"115333","brandKey":"MM"}],"tableTitle":"Materials",":type":"cms-commons/components/content/dynamicproducttable"},"middleresponsivecontainer":{"columnClassNames":{},"cssClassName":"","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--12 aem-Grid--default--12","columns":12,"hasFeatureCard":false,"halfGatedContent":false,"items":[],"allowedComponents":{"applicable":false,"components":[]},"authenticatedUserBGColor":"","mobileSpacing":{},"unauthenticatedUserBGColor":"",":items":{},":itemsOrder":[],"desktopSpacing":{},":type":"cms-commons/components/content/container"},"reference":{"headline":null,"anchorId":null,"startNumber":null,"references":null,":type":"cms-commons/components/content/reference"},"bottomcontainer":{"columnClassNames":{},"cssClassName":"","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--12 aem-Grid--default--12","columns":12,"hasFeatureCard":false,"halfGatedContent":false,"items":[],"allowedComponents":{"applicable":false,"components":[]},"authenticatedUserBGColor":"","mobileSpacing":{},"unauthenticatedUserBGColor":"",":items":{},":itemsOrder":[],"desktopSpacing":{},":type":"cms-commons/components/content/container"}},":itemsOrder":["topcontainer","dynamicproducttable","middleresponsivecontainer","reference","bottomcontainer"],"desktopSpacing":{},":type":"cms-commons/components/content/container"},"rightrailresponsivecontainer":{"columnClassNames":{"cq:responsive":"aem-GridColumn aem-GridColumn--default--3"},"cssClassName":"","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--default--3","columns":12,"hasFeatureCard":false,"halfGatedContent":false,"items":[],"allowedComponents":{"applicable":false,"components":[]},"authenticatedUserBGColor":"","mobileSpacing":{},"unauthenticatedUserBGColor":"",":items":{},":itemsOrder":[],"desktopSpacing":{},":type":"cms-commons/components/content/container"},"footer":{"richText":false,":type":"cms-commons/components/content/footer"}},":itemsOrder":["topresponsivecontainer","utilityresponsivecontainer","bodyresponsivecontainer","rightrailresponsivecontainer","footer"],":type":"wcm/foundation/components/responsivegrid"}},":itemsOrder":["root"],":hierarchyType":"page",":type":"cms-commons/components/structure/page",":path":"/CA/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-the-monoclonal-antibody-mab-erbitux"}