- Penicillin biosynthesis in Aspergillus oryzae and its overproduction by genetic engineering.
Penicillin biosynthesis in Aspergillus oryzae and its overproduction by genetic engineering.
Journal of bioscience and bioengineering (2010-06-15)
Junichiro Marui, Sumiko Ohashi-Kunihiro, Tomohiro Ando, Marie Nishimura, Hideaki Koike, Masayuki Machida
PMID20541108
ABSTRACT
Aspergillus oryzae penicillin biosynthetic genes were clustered. The penicillin production was positively regulated by VeA, a global gene regulator required for transcriptional expression of the penicillin biosynthetic genes. Overexpression of the biosynthetic genes by a strong promoter yielded a greater than 100-fold increase in penicillin production.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
Penicillinase from Bacillus cereus, lyophilized powder, 1,500-3,000 units/mg protein (using benzylpenicillin)