Skip to Content
MilliporeSigma
  • Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Analytical chemistry (2015-03-17)
Evgeni Eltzov, Avital Cohen, Robert S Marks
ABSTRACT

Indoor air pollution became a recent concern found to be oftentimes worse than outdoor air quality. We developed a tool that is cheap and simple and enables continuous monitoring of air toxicity. It is a biosensor with both a nondisposable (monitor) and disposable (calcium alginate pads with immobilized bacteria) elements. Various parameters to enhance its signal have been tested (including the effect of the pad's orientation, it's exposure to either temperature or time with the air toxicant analyte, and various concentrations thereof). Lastly, the sensor has demonstrated its ability to sense the presence of chemicals in a real, indoor environment. This is the first step in the creation of a sensitive and simple operative tool that may be used in different indoor environments.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Sodium chloride solution, 0.9% in water, BioXtra, suitable for cell culture
Sigma-Aldrich
Chloroform, ≥99%, PCR Reagent, contains amylenes as stabilizer
Sigma-Aldrich
Sodium chloride, tablet
Sigma-Aldrich
Sodium chloride, meets analytical specification of Ph. Eur., BP, USP, 99.0-100.5%
Sigma-Aldrich
N,N-Dimethylformamide, for molecular biology, ≥99%
Supelco
Formaldehyde solution, stabilized with methanol, ~37 wt. % in H2O, certified reference material
Sigma-Aldrich
Sodium chloride solution, 5 M
Sigma-Aldrich
Sodium chloride solution, 5 M in H2O, BioReagent, for molecular biology, suitable for cell culture
Supelco
Calcium standard for AAS, analytical standard, 1.000 g/L Ca+2 in hydrochloric acid, traceable to BAM
Sigma-Aldrich
Sodium chloride, BioUltra, for molecular biology, ≥99.5% (AT)
Sigma-Aldrich
N-Ethyldiisopropylamine, BASF quality, ≥98.0%
Sigma-Aldrich
Sodium chloride solution, BioUltra, for molecular biology, ~5 M in H2O
Sigma-Aldrich
Diethylene glycol, puriss. p.a., ≥99.0% (GC), colorless
Sigma-Aldrich
Diethylene glycol, BioUltra, ≥99.0% (GC)
Supelco
(±)-Epichlorohydrin, analytical standard
Sigma-Aldrich
Formaldehyde solution, for molecular biology, BioReagent, ≥36.0% in H2O (T)
Sigma-Aldrich
Tetraethyl orthosilicate, ≥99.0% (GC)
Sigma-Aldrich
Sodium chloride, tested according to Ph. Eur.
Sigma-Aldrich
N-Ethyldiisopropylamine solution, suitable for peptide synthesis, ~2 M in 1-methyl-2-pyrrolidinone
Supelco
1-Octanol, analytical standard
Supelco
Diethylene glycol, analytical standard
Sigma-Aldrich
Formaldehyde solution, meets analytical specification of USP, ≥34.5 wt. %
Sigma-Aldrich
Formaldehyde solution, ACS reagent, 37 wt. % in H2O, contains 10-15% Methanol as stabilizer (to prevent polymerization)
Sigma-Aldrich
Diethyl ether, puriss. p.a., ACS reagent, ≥99.8% (GC)
Sigma-Aldrich
N,N-Dimethylformamide, ACS reagent, ≥99.8%
Sigma-Aldrich
1-Octanol, anhydrous, ≥99%
Sigma-Aldrich
1-Octanol, ≥98%, FCC, FG
Sigma-Aldrich
Formaldehyde solution, for molecular biology, 36.5-38% in H2O
Sigma-Aldrich
(±)-Epichlorohydrin, purum, ≥99% (GC)
Supelco
Tetrahydrofuran, analytical standard