The effect of Ti anodized nano-foveolae structure on preosteoblast growth and osteogenic gene expression.

The effect of Ti anodized nano-foveolae structure on preosteoblast growth and osteogenic gene expression.

Journal of nanoscience and nanotechnology (2014-04-18)

Wei-Qiang Yu, Ling Xu, Fu-Qiang Zhang

ABSTRACT

The TiO2 nanotubes by anodization have been extensively studied for medical implant and orthopedic applications because of enhancing bone development. In the present study, a new nano-foveolae structure verified by SEM and AFM was prepared by simulating the nanotubes exfoliation from anodized Ti. MC3T3-E1 preosteoblasts were used to investigate the effects of new nanoscale surface on cell spreading, proliferation, and osteogenic gene expression. The new nano-foveolae structure supported preosteoblast better spreading, more filopodiaes shown by SEM (4 h), and lower proliferation (72 h) than the smooth Ti. By two weeks, the new nanoscale surface induced higher expression of osteogenic markers alkaline phosphatase (ALP), osteopontin (OPN), osteocalcin (OCN), and collagen I (COL I) with real-time RT-PCR compared to the control group. The results of this study suggest that the new nano-foveolae structure provides a favorable surface for functions of bone cells.

MATERIALS

Product Number

Brand

Product Description

IRMM531B

Titanium, IRMM^®, certified reference material, 0.5 mm foil

IRMM531C

Titanium, IRMM^®, certified reference material, 0.5 mm wire

305812

Sigma-Aldrich

Titanium, 5-10 mm, ≥99.99% trace metals basis (purity exclusive of Na and K content)

267902

Sigma-Aldrich

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266035

Sigma-Aldrich

Titanium, wire, diam. 1.0 mm, 99.99% trace metals basis

369489

Sigma-Aldrich

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460400

Sigma-Aldrich

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348791

Sigma-Aldrich

Titanium, foil, thickness 0.127 mm, 99.7% trace metals basis

348848

Sigma-Aldrich

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Titanium, rod, 25mm, diameter 50mm, 99.99+%

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Titanium, tube, 100mm, outside diameter 6.35mm, inside diameter 5.53mm, wall thickness 0.41mm, annealed, 99.6+%

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Titanium, tube, 1000mm, outside diameter 4.25mm, inside diameter 3.75mm, wall thickness 0.25mm, annealed, 99.6+%

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Titanium, tube, 200mm, outside diameter 2.03mm, inside diameter 1.55mm, wall thickness 0.24mm, annealed, 99.6+%

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