- Large-scale high-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method.
Large-scale high-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method.
Nature protocols (2007-04-03)
R Daniel Gietz, Robert H Schiestl
PMID17401336
ABSTRACT
Here, we describe a Library screen transformation protocol using the lithium acetate/single-stranded carrier DNA/PEG method of transformation for Saccharomyces cerevisiae. This method is suitable for screening complex plasmid libraries such as those used for yeast two-hybrid analysis. This procedure takes up to 2.5 h to complete once the yeast culture has been grown.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
Yeast Nitrogen Base Without Amino Acids, Yeast classification medium used for selecting yeasts based on amino acid and carbohydrate requirements