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Enterotoxigenicity of Staphylococcus intermedius of canine origin.

International journal of food microbiology (1988-12-01)
E Y Hirooka, E E Müller, J C Freitas, E Vicente, Y Yoshimoto, M S Bergdoll
ABSTRACT

Seventy-three staphylococcal strains isolated from pyrodermatitis in dogs were classified as Staphylococcus intermedius (52 strains) or Staphylococcus aureus (21 strains) on the basis of acetoin formation, anaerobic mannitol fermentation, aerobic maltose fermentation, pigmentation, coagulation of human plasma, and reaction on crystal violet agar. Enterotoxin was produced by 13 of the 52 S. intermedius strains and 6 of the S. aureus strains. The highest percentage of enterotoxigenic strains produced enterotoxins C (6 strains), D (7 strains), and E (6 strains). Four strains produced the toxic shock syndrome toxin-1. There was little difference in the antibiotic susceptibility between the enterotoxigenic and non-enterotoxigenic strains.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Acetoin, natural, ≥95%, FG
Supelco
Acetoin, analytical standard
Sigma-Aldrich
Acetoin, May exist as crystalline dimer
Sigma-Aldrich
Acetoin, primarily dimer, ≥95%, FG