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  • Isotachophoresis as a candidate reference method in analytical chemistry. Determination of sodium in serum.

Isotachophoresis as a candidate reference method in analytical chemistry. Determination of sodium in serum.

Journal of chromatography (1985-02-22)
A A Lemmens, J C Reijenga, F M Everaerts, R T Janssen, J A Hulsman, C A Meijers
ABSTRACT

Isotachophoresis seems a likely candidate for a reference method, as it is more accurate and precise than common routine methods. The response is highly linear and depends on a well defined transport number of the leading ion, stability of the driving current, mobility of the separand, which is well controlled by the leading electrolyte and the use of a high-resolution detector. The suitability of isotachophoresis as a reference method was investigated for the determination of sodium in human serum. The operational conditions were 0.01 M K+/citrate (leading electrolyte) at pH 5.5 and 0.01 M creatinine X HCl (terminating electrolyte). Both n-butylamine and ammediol could be used as internal standards. The calibration graph constructed from standard solutions, diluted by weight with the internal standard, yielded a correlation coefficient of 0.99994 (n = 50) in the working range. The method seems especially useful for the determination of any ionic solute in, e.g., clinical samples (lithium, calcium, creatinine or drugs).

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
2-Amino-2-methyl-1,3-propanediol, BioUltra, ≥99.5% (NT)
Sigma-Aldrich
2-Amino-2-methyl-1,3-propanediol, ≥99%