- 5-Fluoroorotic acid as a selective agent in yeast molecular genetics.
5-Fluoroorotic acid as a selective agent in yeast molecular genetics.
Methods in enzymology (1987-01-01)
J D Boeke, J Trueheart, G Natsoulis, G R Fink
PMID3323810
ABSTRACT
5-FOA is an extremely useful reagent for the selection of Ura- cells amid a population of Ura+ cells. The selection is effective in transformation and recombination studies where loss of URA3+ is desired. A new plasmid shuffling procedure based on the 5-FOAR selection permits the recovery of conditional lethal mutations in cloned genes that encode vital functions.
MATERIALS