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Thiazole orange: a new dye for reticulocyte analysis.

Cytometry (1986-11-01)
L G Lee, C H Chen, L A Chiu
ABSTRACT

The purpose of this study was to find a 488-nm excitable fluorescent dye for reticulocyte analysis by the use of fluorescence activated cell cytometry. The chemical structure of thioflavin T, a dye used for reticulocyte analysis with 457-nm excitation, was used as a model. Several dyes were synthesized and evaluated by quantum yield determination, fluorescence microscopy, and flow cytometry. The best results were obtained with a dye we have named "thiazole orange"; analysis of several blood samples with thiazole orange gave a correlation coefficient of 0.97 as compared to a manual determination of reticulocyte percentage.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Thiazole Orange, Dye content ~90 %
Sigma-Aldrich
SYBR® Green II RNA gel stain, 10,000 × in DMSO