- Actin chromobody imaging reveals sub-organellar actin dynamics.
Actin chromobody imaging reveals sub-organellar actin dynamics.
Nature methods (2020-08-12)
Cara R Schiavon, Tong Zhang, Bing Zhao, Andrew S Moore, Pauline Wales, Leonardo R Andrade, Melissa Wu, Tsung-Chang Sung, Yelena Dayn, Jasmine W Feng, Omar A Quintero, Gerald S Shadel, Robert Grosse, Uri Manor
PMID32778832
ABSTRACT
The actin cytoskeleton plays multiple critical roles in cells, from cell migration to organelle dynamics. The small and transient actin structures regulating organelle dynamics are challenging to detect with fluorescence microscopy, making it difficult to determine whether actin filaments are directly associated with specific membranes. To address these limitations, we developed fluorescent-protein-tagged actin nanobodies, termed 'actin chromobodies' (ACs), targeted to organelle membranes to enable high-resolution imaging of sub-organellar actin dynamics.
MATERIALS
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Anti-FIS1 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution