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Plasmid DNA Purification

Bacterial plasmid DNA purification for molecular cloning, transcription, transformation, recombinant protein expression, and PCR applications

Plasmid purification is commonly used for molecular cloning, transformation, and recombinant protein expression. Plasmid purification kits are available based on size of the bacterial culture and corresponding plasmid yield (miniprep, midiprep, maxiprep, megaprep, and gigaprep).  

Our plasmid kits provide reliable isolation of high quality plasmid DNA. These kits are:

  • Designed with advanced silica bind and elute technology
  • Compatible with both vacuum and spin format
  • Formulated without phenol/chloroform

These products allow researchers to maximize the reproducibility of their workflow and minimize their time spent on obtaining plasmid DNA for transfection, sequencing, PCR, and other downstream applications. 

GenElute™ plasmid miniprep kits

GenElute™ plasmid miniprep kits offer simple, rapid, and cost-effective methods for isolating plasmid DNA from E. coli cultures. These kits combine silica-based membrane technology and the convenience of a spin column format.

Features:

  • 40% more preps per kit than the leading supplier
  • Purify up to 20 µg of plasmid DNA per mL of culture
  • Purified plasmid DNA in less than 30 minutes for up to 24 preps
  • No phenol/chloroform extraction or alcohol precipitation required

GenElute™ HP plasmid prep kits

GenElute™ HP plasmid purification kits yield high quality plasmid DNA in less than 30 minutes for Mini, Midi, and Maxiprep kits, or 1.5 hours for Mega and Gigaprep kits.

  • From harvested bacterial culture to pure plasmid DNA in less time
  • Up to 25 µg (Mini), 350 µg (Midi), 1.2 mg (Maxi), 5 mg (Mega), and 15 mg (Giga) yield of high-copy plasmid DNA
  • Offers the flexibility of a vacuum or spin format (Mega and Gigaprep in vacuum format only)
  • No phenol/chloroform extraction or alcohol precipitation required
  • Kits are stable at room temperature for convenient storage

GENOPURE™ PLASMID PREP KITS

Roche Genopure™ midi and maxi prep kits use anion exchange chromatography for plasmid purification. A modified alkaline lysis protocol optimized for cell cultures grown in LB media is used to isolate DNA. The lysate can be filtered rather than centrifuged prior to anion exchange, resulting in shorter plasmid prep times and complete removal of SDS from the purified product.

  • Reduce DNA shearing by clarifying lysate using pre-folded filters rather than centrifugation prior to anion exchange.
  • Save time with ready-to-use reagents (10 minutes hands-on-time/75 minutes overall).
  • Purify all sizes and types of plasmid, even BAC DNA.
  • Process multiple samples in parallel using high speed gravity-flow columns.
  • Eliminate the use of hazardous organic compounds such as cesium chloride, phenol, chloroform, and ethidium bromide.

GenElute™ endotoxin-free plasmid prep kits

Endotoxins reduce cell viability and transfection efficiencies among different eukaryotic cell lines and can influence the outcome and reproducibility of a given transfection experiment when using mammalian and other eukaryotic cells. GenElute™ endotoxin-free plasmid prep kits offer a simple, rapid, cost-effective method for purifying plasmid DNA with <0.1

EU/mg DNA for high-efficiency transfection.

  • Higher transfection efficiency and yield than market leaders
  • Purify endotoxin-free plasmid DNA (≤0.1 EU/mg DNA)

GenElute™ five-minute plasmid miniprep kits

GenElute™ five-minute miniprep kits feature a streamlined protocol yielding up to 5 mg high-quality plasmid DNA in about five minutes.

  • Only 5 minutes from start to finish
  • No pelleting cells or clearing lysates
  • Binding column works with any standard laboratory vacuum manifold
  • Choose vacuum or spin protocols

Related Product Resources

Product Guide: Nucleic Acid Purification
Article: Plasmid DNA Purification
Article: DNA & RNA Purification

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GenElute™ Five-Minute Plasmid Miniprep procedure
GenElute™ Five-Minute Plasmid Miniprep procedure
The GenElute™ Five-Minute Plasmid Miniprep kit features an ultra streamlined protocol yielding high-quality plasmid DNA in about five minutes. The DNA is suitable for immediate use in capillary DNA sequencing, clone screening, restriction digestion and PCR.
GenElute™ HP Plasmid Maxiprep Procedure
GenElute™ HP Plasmid Maxiprep Procedure
The GenElute HP Plasmid Maxiprep kit offers a simple, rapid, and cost-effective method for isolating plasmid DNA from recombinant E. coli cutures. The recovered plasmid DNA is ready for immediate use in downstream applications, such as restriction digestion, ligation, sequencing, PCR, transformation, and transfection.
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