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T8575

Sigma-Aldrich

Anti-Tryptophan Hydroxylase antibody produced in sheep

affinity isolated antibody, buffered aqueous glycerol solution

Synonym(s):

Anti-TPH

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About This Item

MDL number:
MFCD00282500
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

sheep

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous glycerol solution

usage

sufficient for 10 blots

species reactivity

mammals, human

technique(s)

western blot: 1:1,000 using human dorsal raphe nucleus

UniProt accession no.

P17752

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... TPH1(7166)
mouse ... Tph1(21990)
rat ... Tph1(24848)

General description

The tryptophan hydroxylase 1 (TPH1) gene spanning 29kbp with 11 exons is mapped to human chromosome 11p15.3-p14. TPH1, is a 444 amino acid protein expressed in the gut, spleen, thymus and also in the pineal gland and the pituitary.
Tryptophan Hydroxylase (TPH) is a member of the aromatic amino acid hydroxylases (AAAH) family.

Immunogen

recombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E. coli and purified by preparative SDS-PAGE.

Application

Anti-Tryptophan Hydroxylase antibody produced in sheep has been used in:
  • immunocytochemistry
  • immunohistochemistry
  • immunofluorescence
  • immunoperoxidase staining

Biochem/physiol Actions

The tryptophan hydroxylase 1 gene (TPH1) catalyzes the synthesis of 5-hydroxytryptophan, which is a precursor to the neurotransmitter serotonin. Variation in the gene expression affects prefrontal cortex activation during response inhibition. Mutation in the TPH1 gene leads to schizophrenia, irritable bowel syndrome (IBS) in women, suicidal behavior and depressive disorders. The encoded protein plays a vital role in regulation of cardiovascular function.

Physical form

Solution in 150 mM NaCl, 10 mM HEPES, pH 7.5, 100 μg per mL BSA, and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Kristina M Wright et al.
eLife, 8 (2019-03-08)
Faced with potential harm, individuals must estimate the probability of threat and initiate an appropriate fear response. In the prevailing view, threat probability estimates are relayed to the ventrolateral periaqueductal gray (vlPAG) to organize fear output. A straightforward prediction is
TPH1 A218C polymorphism and temperament in major depression.
Andre K
BMC Psychiatry (2013)
B M Spannuth et al.
Neuroscience, 179, 104-119 (2011-02-01)
Serotonergic systems are thought to play an important role in control of motor activity and emotional states. We used a fear-potentiated startle paradigm to investigate the effects of a motor-eliciting stimulus in the presence or absence of induction of an
Matthew W Hale et al.
Psychopharmacology, 213(2-3), 243-264 (2010-11-20)
Dysfunction of serotonergic systems is thought to play an important role in a number of neurological and psychiatric disorders. Recent studies suggest that there is anatomical and functional diversity among serotonergic systems innervating forebrain systems involved in the control of
TPH gene polymorphisms are associated with disease perception and quality of life in women with irritable bowel syndrome.
Jun SE
Biological Research for Nursing, 16, 95-104 (2014)
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