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GE27-5340-01

AutoSeq G-50

Cytiva 27-5340-01, pack of 50 columns

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About This Item

UNSPSC Code:
41105501
NACRES:
NA.52

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

packaging

pack of 50 columns

manufacturer/tradename

Cytiva 27-5340-01

particle size

50-150 μm (dry)

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General description

illustra Autoseq G-50 columns are specifically designed to remove fluorescent dye-terminators from sequencing reactions prior to analysis on automated sequencers. Effective purification is essential for high-quality sequencing results.

Application

illustra Autoseq G-50 columns are specifically designed to remove fluorescent dye-terminators from sequencing reactions prior to analysis on automated sequencers. Effective purification is essential for high-quality sequencing results since residual dye-terminators can obscure data.

llustra Autoseq G-50 consists of MicroSpin columns containing Sephadex® G-50, pre-equilibrated in double-distilled water. This is important because even the small amount of salt in traditional buffers (such as TE buffer) can cause electrophoretic artifacts on salt-sensitive automated sequencers.

illustra Autoseq G-50 columns can be used to clean up reactions prepared with the BigDye Terminator v3.1 Cycle Sequencing Kit from Applied Biosystems.

Features and Benefits

  • For rapid removal of unincorporated fluorescent dye-terminators from cycle sequencing reactions prior to automated sequencing.
  • Columns are prepacked with Sephadex® G-50 DNA Grade F and pre-equilibrated in double-distilled water with 0.05% Kathon CG/ICP Biocide added as a preservative.
  • Columns that are ready to use enable sample application to collection of purified product in less than 4 min.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

AutoSeq is a trademark of Cytiva

Storage Class Code

12 - Non Combustible Liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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