GE27-5340-01
AutoSeq™ G-50
Cytiva 27-5340-01, pack of 50 columns
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shelf life
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging
pack of 50 columns
manufacturer/tradename
Cytiva 27-5340-01
particle size
50-150 μm (dry)
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General description
illustra™ Autoseq™ G-50 columns are specifically designed to remove fluorescent dye-terminators from sequencing reactions prior to analysis on automated sequencers. Effective purification is essential for high-quality sequencing results.
Application
illustra™ Autoseq™ G-50 columns are specifically designed to remove fluorescent dye-terminators from sequencing reactions prior to analysis on automated sequencers. Effective purification is essential for high-quality sequencing results since residual dye-terminators can obscure data.
llustra Autoseq™ G-50 consists of MicroSpin™ columns containing Sephadex® G-50, pre-equilibrated in double-distilled water. This is important because even the small amount of salt in traditional buffers (such as TE buffer) can cause electrophoretic artifacts on salt-sensitive automated sequencers.
illustra™ Autoseq™ G-50 columns can be used to clean up reactions prepared with the BigDye Terminator v3.1 Cycle Sequencing Kit from Applied Biosystems.
llustra Autoseq™ G-50 consists of MicroSpin™ columns containing Sephadex® G-50, pre-equilibrated in double-distilled water. This is important because even the small amount of salt in traditional buffers (such as TE buffer) can cause electrophoretic artifacts on salt-sensitive automated sequencers.
illustra™ Autoseq™ G-50 columns can be used to clean up reactions prepared with the BigDye Terminator v3.1 Cycle Sequencing Kit from Applied Biosystems.
Features and Benefits
- For rapid removal of unincorporated fluorescent dye-terminators from cycle sequencing reactions prior to automated sequencing.
- Columns are prepacked with Sephadex® G-50 DNA Grade F and pre-equilibrated in double-distilled water with 0.05% Kathon CG/ICP Biocide added as a preservative.
- Columns that are ready to use enable sample application to collection of purified product in less than 4 min.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
AutoSeq is a trademark of Cytiva
Storage Class Code
12 - Non Combustible Liquids
Certificates of Analysis (COA)
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