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biological source
mouse nerve
growth mode
Adherent
karyotype
modal no. of chromosomes is 192 see reference
morphology
neuronal
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Mouse neuroblastoma
Cell Line Description
A subclone of the cell line N1E-115 established by Amano et al. The cell line N1E-115 is an adrenergic cell line derived from mouse neuroblastoma C1300. (Mouse strain A/Jax). N1E-115 cells are reported to be slightly contaminated with with a cell type of the morphology of C6 glioma cells. It is recommended to use N1E-115-1, a subclone of N1E-115, that is devoid of the contamination.
Application
can be used as a neuronal model
Culture Medium
DMEM + 2 mM Glutamine + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.05% trypsin/EDTA; 5% CO2; 37°C. Population doubling approximately 20 hours.
Other Notes
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Certificates of Analysis (COA)
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