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51449C

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Dulbecco′s Modified Eagle′s Medium/High Modified

with 4500 mg/L dextrose, with 110mg/L sodium pyruvate, without L-glutamine, without phenol red, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Dulbecco’s Modified Eagle’s Medium - high glucose, DME, DMEM

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

description

for research or for further manufacturing use

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable
cell culture | stem cell: suitable

components

phenol red: no
glucose: high
sodium pyruvate: yes
L-glutamine: no

shipped in

ambient

storage temp.

2-8°C

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Application

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additonally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High". Dulbecco′s Modified Eagle′s Medium/High Modified has been used as a supplement in cell culture.
Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Whole-cell Escherichia coli lactate biosensor for monitoring mammalian cell cultures during biopharmaceutical production
Geors L, et al.
Biotechnology and Bioengineering, 114(6), 1290-1300 (2017)

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