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Key Documents

AB5042

Sigma-Aldrich

Anti-Choline Acetyltransferase Antibody

serum, Chemicon®

Synonym(s):

ChAT

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

guinea pig, rat, avian

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

chicken ... Chat(395314)
rat ... Chat(290567)

Specificity

Will stain cholinergic neurons in rat central and peripheral nervous systems.

Immunogen

A 22 amino acid synthetic peptide from porcine ChAT, coupled to KLH. The peptide sequence is GLFSSYRLPGHTQDTLVAQKSS. Due to sequence similarity, the antibody is expected to react with pig, rat, mouse and human ChAT.

Application

Detect Choline Acetyltransferase using this Anti-Choline Acetyltransferase Antibody validated for use in IH.
Immunohistochemistry: 1:2,000-1:4,000 in rat basal forebrain.

Western blot

Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors

Neuronal & Glial Markers

Physical form

Lyophilized. Reconstitute with 100 μL of sterile distilled water. Contains no preservative.

Storage and Stability

Maintain lyophilized material dry at -20°C or -70°C for up to 6 months after date of receipt. After reconstitution, maintain at -20°C in undiluted aliquots for up to 6 months. Glycerol (1:1) can be added for additional stability.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Acetylcholine as an age-dependent non-neuronal source in the heart.
Obaida R Rana,Patrick Schauerte,Rahel Kluttig,Jorg W Schroder,Rory R Koenen et al.
Autonomic Neuroscience : Basic & Clinical null
C Brodski et al.
Proceedings of the National Academy of Sciences of the United States of America, 97(17), 9683-9688 (2000-08-10)
Neurotrophins influence the epigenetic shaping of the vertebrate nervous system by regulating neuronal numbers during development and synaptic plasticity. Here we attempt to determine whether these growth factors can also regulate neurotransmitter plasticity. As a model system we used the
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Morrey, JD; Siddharthan, V; Wang, H; Hall, JO; Skirpstunas, RT; Olsen, AL; Nordstrom et al.
Journal of Neurovirology null
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Amyotrophic lateral sclerosis (ALS) is a progressively fatal, incurable, neurodegenerative disorder. In this study, we investigated whether olfactory ensheathing cells (OEC) transplantation could provide protection to motor neurons and enable remyelination in mutant SOD1(G93A) transgenic rats with ALS. Seventy-two rats
Persistent west nile virus associated with a neurological sequela in hamsters identified by motor unit number estimation.
Venkatraman Siddharthan, Hong Wang, Neil E Motter, Jeffery O Hall, Robert D Skinner et al.
Journal of virology null

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