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393207-M

Millipore

Anti-HNE-Michael Adducts, Reduced Rabbit pAb

liquid, Calbiochem®

Synonym(s):

Anti-4-Hydroxy-2-noneal Michael Adducts, Reduced

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About This Item

UNSPSC Code:
12352203

biological source

rabbit

Quality Level

100

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

form

liquid

does not contain

preservative

species reactivity (predicted by homology)

all

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG

storage temp.

−70°C

target post-translational modification

unmodified

General description

Anti-HNE-Michael Adducts, Reduced, rabbit polyclonal, specifically recognizes chemically reduced amino acid-(4)-HNE adducts. It is validated for use in ELISA, WB & IHC (frozen & paraffin sections).
Rabbit polyclonal antibody supplied as diluted serum. Recognizes, specifically, chemically reduced amino acid-(4)-HNE adducts.
Recognizes chemically reduced amino acid-(4)-HNE adducts. Does not recognize non-reduced adducts or reduced modifications arising from malondialdehyde (MDA) and structurally-related alkenes such as trans-2-noneal.
The CNS is enriched in polyunsaturated fatty acids and is therefore particularly vulnerable to lipid peroxidation. 4-Hydroxy-2-noneal (HNE) is one of the major lipid peroxidation products with cytotoxic and mutagenic activity. It reacts with protein residues such as histidine to generate stable Michael adducts. HNE has been shown to inhibit neurite outgrowth and disrupt microtubules and tubulin modification, suggesting it may play a role in Alzheimer′s disease.

Specificity

A broad range of species

Immunogen

HNE-treated KLH in which 1:1 amino acid-HNE Michael adducts were chemically reduced

Application

ELISA (1:500-1:1000)

Immunoblotting (1:1000-1:4000)

Frozen Sections (1:50-1:500)

Paraffin Sections (1:50-1:500)

Warning

Toxicity: Standard Handling (A)

Physical form

Serum diluted in 50% glycerol, pH 7.5.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Does not bind non-reduced adducts and reduced modifications arising from malondialdehyde (MDA) or structurally-related alkenals such as trans-2-noneal. Does not cross-react with non-reduced amino acid-HNE Michael adducts. Antibody binding requires the presence of the 4-hydroxyl group. Variables associated with assay conditions will dictate the optimal working dilution.
Toyokuni, S., et al. 2000. Antioxid. Redox. Signal2, 681.
Neely, M.D., et al. 1999. J. Neurochem.72, 2323.
Uchida, K., et al. 1995. Biochem. Biophys. Res. Commun.212, 1068.
Uchida, K., et al. 1995. Arch. Biochem. Biophys.324, 241.
Uchida, K., et al. 1994. Biochemistry33, 12487.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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