05-636-AF647
Anti-phospho Histone H2A.X (Ser139) Antibody, clone JBW301, Alexa Fluor™ 647
clone JBW301, 0.5 mg/mL, from mouse
Synonym(s):
Histone H2AX, H2a/x, Histone H2A.X
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
mouse
Quality Level
conjugate
ALEXA FLUOR™ 647
antibody form
purified antibody
antibody product type
primary antibodies
clone
JBW301, monoclonal
species reactivity
human
species reactivity (predicted by homology)
vertebrates (based on 100% sequence homology)
concentration
0.5 mg/mL
technique(s)
immunocytochemistry: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (pSer139)
Gene Information
human ... H2AX(3014)
General description
As a member of the histone H2A family, histone H2A.x (H2A.x) is a variant histone H2A which replaces conventional H2A in a subset of nucleosomes. H2A.x is involved in the DNA repair of double-strand breakage (DSB) damage on nuclear DNA. After a double strand DNA break, H2A.x is rapidly phosphorylated at serine 139 by ATM kinase and becomes gamma-H2AFX. This phosphorylation step can extend up to several thousand nucleosomes from the actual site of the DSB and may mark the surrounding chromatin for recruitment of proteins required for DNA damage signaling and repair. As a part of posttranslational modifications during apoptosis caused by severe DNA damage, high expression of phosphorylated H2A.x is considered as an accurate indicator of apoptosis.
Immunogen
Linear peptide corresponding to phospho Histone H2A.X (Ser139).
Application
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
This Anti-phospho Histone H2A.X (Ser139) Antibody, clone JBW301, Alexa Fluor™ 647 is validated for use in ICC for the detection of phospho Histone H2A.X (Ser139).
Quality
Evaluated by Immunocytochemistry in untreated and staurosporin treated HeLa cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected phospho Histone H2A.X (Ser139) in staurosporin treated HeLa cells.
Alexa Fluor™ is a registered trademark of Life Technologies.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected phospho Histone H2A.X (Ser139) in staurosporin treated HeLa cells.
Alexa Fluor™ is a registered trademark of Life Technologies.
Target description
~17 kDa observed. Refer to Cat. No. 05-636 for observed molecular weight information.
Physical form
Protein G Purified
Purified mouse conjugate in buffer containing PBS, 15 mg/ml BSA and 0.1% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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