Pular para o conteúdo
MilliporeSigma

Phasor Representation of Monomer-Excimer Kinetics: General Results and Application to Pyrene.

The journal of physical chemistry. B (2015-11-10)
Liliana Martelo, Alexander Fedorov, Mário N Berberan-Santos
RESUMO

Phasor plots of the fluorescence intensity decay (plots of the Fourier sine transform versus the Fourier cosine transform, for one or several angular frequencies) are being increasingly used in studies of homogeneous and heterogeneous systems. In this work, the phasor approach is applied to monomer-excimer kinetics. The results obtained allow a clear visualization of the information contained in the decays. The monomer phasor falls inside the universal circle, whereas the excimer phasor lies outside it, but within the double-exponential outer boundary curve. The monomer and excimer phasors, along with those corresponding to the two exponential components of the decays, fall on a common straight line and obey the generalized lever rule. The clockwise trajectories described by both phasors upon monomer concentration increase are identified. The phasor approach allows discussing in a single graphic not only the effect of concentration but also that of rate constants, including the evolution from irreversible kinetics to fast excited-state equilibrium upon a temperature increase. The obtained results are applied to the fluorescence decays of pyrene monomer and excimer in methylcyclohexane at room temperature. A straightforward method of monomer-excimer lifetime data analysis based on linear plots is also introduced.

MATERIAIS
Número do produto
Marca
Descrição do produto

Sigma-Aldrich
Methylcyclohexane, ReagentPlus®, 99%
SKU
Tamanho da embalagem
Disponibilidade
Preço
Quantidade
Sigma-Aldrich
Pyrene, 98%
SKU
Tamanho da embalagem
Disponibilidade
Preço
Quantidade
Sigma-Aldrich
1-Hexanethiol, 95%
SKU
Tamanho da embalagem
Disponibilidade
Preço
Quantidade
Sigma-Aldrich
Pyrene, puriss. p.a., for fluorescence, ≥99.0% (GC)
SKU
Tamanho da embalagem
Disponibilidade
Preço
Quantidade
Sigma-Aldrich
Methylcyclohexane, spectrophotometric grade, 99%
SKU
Tamanho da embalagem
Disponibilidade
Preço
Quantidade
Supelco
Pyrene, analytical standard
SKU
Tamanho da embalagem
Disponibilidade
Preço
Quantidade
Sigma-Aldrich
Methylcyclohexane, anhydrous, ≥99%
SKU
Tamanho da embalagem
Disponibilidade
Preço
Quantidade
Sigma-Aldrich
1-Hexanethiol, 99% (GC)
SKU
Tamanho da embalagem
Disponibilidade
Preço
Quantidade
Sigma-Aldrich
Pyrene, sublimed grade, 99%
SKU
Tamanho da embalagem
Disponibilidade
Preço
Quantidade